Relevance of control diet choice in metabolic studies: impact in glucose homeostasis and vascular function
Relevance of control diet choice in metabolic studies: impact in glucose homeostasis and vascular function
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DOI：
10.1038/s41598-020-59674-0
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发表时间：
2020-02-19
影响因子：
4.6
通讯作者：
Somoza, Beatriz
中科院分区：
文献类型：
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作者：
Gonzalez-Blazquez, Raquel;Alcala, Martin;Somoza, Beatriz
研究心脏代谢紊乱的实验方法需要使用用商业饮食喂养的动物模型，商业饮食的组成明显不同，甚至在用于对照组的饮食之间也是如此。虽然普通饮食通常由农业副产品制成，但纯化的低脂饮食（LF）含有更高百分比的易代谢碳水化合物，以及减少的多不饱和脂肪酸，微量营养素和纤维，所有这些都与代谢和血管功能障碍有关。我们假设，LF饮食，通常用于控制动物，可以促进不良的血管和代谢结果。为了解决这个问题，5周龄的雄性C57BL6J小鼠用标准（Chow）或LF饮食喂养6周。记录体重、肥胖、生化参数、全身和主动脉胰岛素敏感性和内皮功能的变化。LF饮食没有改变体重，但显著损害全身葡萄糖耐量，并增加甘油三酯和胆固醇水平。LF组内皮功能和主动脉胰岛素敏感性显著受损，这是由于NO可用性降低。这些发现强调了在代谢研究中选择适当对照饮食的重要性。这也可能表明，在使用LF作为对照饮食的实验研究中获得的一些心脏代谢变化可能被低估。
The experimental approach for the study of cardiometabolic disorders requires the use of animal models fed with commercial diets whose composition differs notably, even between diets used for control groups. While chow diets are usually made of agricultural by-products, purified low-fat diets (LF) contain a higher percentage of easy metabolizable carbohydrates, together with a reduced amount of polyunsaturated fatty acids, micronutrients and fiber, all associated with metabolic and vascular dysfunction. We hypothesize that the LF diet, commonly used in control animals, could promote adverse vascular and metabolic outcomes. To address this issue, 5-week-old male C57BL6J mice were fed with a standard (Chow) or a LF diet for 6 weeks. Changes in body weight, adiposity, biochemical parameters, systemic and aortic insulin sensitivity and endothelial function were recorded. LF diet did not modify body weight but significantly impaired systemic glucose tolerance and increased triglycerides and cholesterol levels. Endothelial function and aortic insulin sensitivity were significantly impaired in the LF group, due to a reduction of NO availability. These findings highlight the importance of selecting the proper control diet in metabolic studies. It may also suggest that some cardiometabolic alterations obtained in experimental studies using LF as a control diet may be underestimated.