Nucleosome positioning by the winged helix transcription factor HNF3
Nucleosome positioning by the winged helix transcription factor HNF3
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DOI：
10.1101/gad.12.1.5
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发表时间：
1998-01-01
影响因子：
10.5
通讯作者：
Zaret, KS
中科院分区：
文献类型：
--
作者：
Shim, EY;Woodcock, C;Zaret, KS
核小体在基因调控序列上的定位还不清楚。小鼠血清白蛋白基因的转录增强子在肝脏中是活跃的，其中调节因子占据它们在命名为N1、N2和N3的三个核小体样颗粒上的靶位点。翼螺旋转录因子HNF 3结合到N1颗粒中心附近的两个位点。我们使用白蛋白增强子序列创建了双核小体模板，并发现HNF 3蛋白的位点特异性结合导致体外核小体定位与肝细胞核中所见相似。因此，转录因子的结合可以定位潜在的核小体核心。
Nucleosome positioning at genetic regulatory sequences is not well understood. The transcriptional enhancer of the mouse serum albumin gene is active in liver, where regulatory factors occupy their target sites on three nucleosome-like particles designated N1, N2, and N3. The winged helix transcription factor HNF3 binds to two sites near the center of the N1 particle. We created dinucleosome templates using the albumin enhancer sequence and found that site-specific binding of HNF3 protein resulted in nucleosome positioning in vitro similar to that seen in liver nuclei. Thus, binding of a transcription factor can position an underlying nucleosome core.