PEGylated polyplex micelles from triblock catiomers with spatially ordered layering of condensed pDNA and buffering units for enhanced intracellular gene delivery
PEGylated polyplex micelles from triblock catiomers with spatially ordered layering of condensed pDNA and buffering units for enhanced intracellular gene delivery
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DOI：
10.1021/ja0440506
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发表时间：
2005-03-09
影响因子：
15
通讯作者：
Kataoka, K
中科院分区：
文献类型：
--
作者：
Fukushima, S;Miyata, K;Kataoka, K
一种A-B-C型三嵌段共聚物，在一条聚合物链中串联排列两种不同pKa值的聚阳离子，用于构建新型的聚合物胶束，满足高DNA凝聚能力以及针对提高基因转染的质子缓冲活性。胶束可能具有独特的三层结构，其中具有聚（l-赖氨酸）（pKa <9.4）作为C片段的缩合pDNA的内部聚合复合物层被具有相对低的pKa <6.2的聚[（3-吗啉代丙基）N-异丙基酰胺]（B片段）的中间层连续包裹以提供缓冲作用，并且外部PEG层（A片段）作为生物相容性栅栏。
An A−B−C type triblock copolymer, tandemly aligning two types of polycations with different pKavalues in a single polymer strand, was developed for the construction of novel polyplex micelles, satisfying a high DNA condensing ability as well as a proton buffering activity directed to elevating gene transfection. The micelle might feature the distinctive three-layered structure, where an inner polyplex layer of condensed pDNA with poly(l-lysine) (pKa∼9.4) as the C segment is successively wrapped with an intermediate layer of poly[(3-morpholinopropyl)aspartamide] (B segment) with a comparatively low pKaof ∼6.2, to provide a buffering effect, and an outer PEG layer (A segment) as a biocompatible palisade.