Copy number variation affecting the Photoperiod-B1 and Vernalization-A1 genes is associated with altered flowering time in wheat (Triticum aestivum).
Copy number variation affecting the Photoperiod-B1 and Vernalization-A1 genes is associated with altered flowering time in wheat (Triticum aestivum).
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DOI：
10.1371/journal.pone.0033234
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Laurie DA
中科院分区：
文献类型：
--
作者：
Díaz A;Zikhali M;Turner AS;Isaac P;Laurie DA
一年中开花的时间是影响植物繁殖成功的重要适应性特征，并且对作物产量至关重要。开花时间已被广泛地操纵在作物如小麦（Triticum aestivum L.）在驯化过程中，这使它们能够在广泛的环境中生产性地生长。控制开花时间的几个主效基因已经在小麦中被鉴定出具有突变等位基因，这些突变等位基因具有序列变化，如插入、缺失或点突变。我们研究了通过改变光周期反应（Ppd-B1等位基因）或春化要求（Vrn-A1等位基因）调节开花的小麦商业品种的遗传变异，并且在基因序列中没有发现候选突变。遗传学和基因组学方法表明，在这两种情况下，赋予改变开花时间的等位基因具有增加的基因拷贝数和改变的基因表达。Ppd-B1拷贝数增加的等位基因赋予早开花日中性表型，并独立出现至少两次。Vrn-A1拷贝数增加的植物对春化作用的需求增加，因此需要更长时间的寒冷来促进开花。结果表明，拷贝数变异（CNV）在小麦适应中起着重要作用。
The timing of flowering during the year is an important adaptive character affecting reproductive success in plants and is critical to crop yield. Flowering time has been extensively manipulated in crops such as wheat (Triticum aestivum L.) during domestication, and this enables them to grow productively in a wide range of environments. Several major genes controlling flowering time have been identified in wheat with mutant alleles having sequence changes such as insertions, deletions or point mutations. We investigated genetic variants in commercial varieties of wheat that regulate flowering by altering photoperiod response (Ppd-B1 alleles) or vernalization requirement (Vrn-A1 alleles) and for which no candidate mutation was found within the gene sequence. Genetic and genomic approaches showed that in both cases alleles conferring altered flowering time had an increased copy number of the gene and altered gene expression. Alleles with an increased copy number of Ppd-B1 confer an early flowering day neutral phenotype and have arisen independently at least twice. Plants with an increased copy number of Vrn-A1 have an increased requirement for vernalization so that longer periods of cold are required to potentiate flowering. The results suggest that copy number variation (CNV) plays a significant role in wheat adaptation.
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影响因子：
64.8
作者：
Gan X;Stegle O;Behr J;Steffen JG;Drewe P;Hildebrand KL;Lyngsoe R;Schultheiss SJ;Osborne EJ;Sreedharan VT;Kahles A;Bohnert R;Jean G;Derwent P;Kersey P;Belfield EJ;Harberd NP;Kemen E;Toomajian C;Kover PX;Clark RM;Rätsch G;Mott R
通讯作者：
Mott R
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
12.3
作者：
Saintenac C;Jiang D;Akhunov ED
通讯作者：
Akhunov ED
影响因子：
5.4
作者：
Börner, A;Schumann, E;Weber, WE
通讯作者：
Weber, WE
影响因子：
1.9
作者：
Mohler, V;Lukman, R;William, M
通讯作者：
William, M