Emerging approaches to probing ion channel structure and function
Emerging approaches to probing ion channel structure and function
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DOI：
10.1007/s12264-012-1248-0
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发表时间：
2012-07
影响因子：
5.6
通讯作者：
Wei-Guang Li;Tian-Le Xu
中科院分区：
文献类型：
--
作者：
Wei-Guang Li;Tian-Le Xu
离子通道作为细胞膜蛋白，是细胞的感受器。它们作为与质膜以外世界的第一条通信线路，将外部和内部环境的变化转化为独特的电信号，以塑造可兴奋细胞的反应。由于离子通道在细胞通讯中的重要性，人们在结构和功能水平上对离子通道进行了深入的研究。在这里，我们总结了不同的方法，包括分子和细胞，化学，光学，生物物理和计算，用于探测通道激活（或敏化），失活（或脱敏），和各种形式的调制过程中发生的结构和功能重排。通过多学科方法对离子通道的结构和功能的新见解允许开发新的药物疗法以及用于控制细胞活性的新工具。
Ion channels, as membrane proteins, are the sensors of the cell. They act as the first line of communication with the world beyond the plasma membrane and transduce changes in the external and internal environments into unique electrical signals to shape the responses of excitable cells. Because of their importance in cellular communication, ion channels have been intensively studied at the structural and functional levels. Here, we summarize the diverse approaches, including molecular and cellular, chemical, optical, biophysical, and computational, used to probe the structural and functional rearrangements that occur during channel activation (or sensitization), inactivation (or desensitization), and various forms of modulation. The emerging insights into the structure and function of ion channels by multidisciplinary approaches allow the development of new pharmacotherapies as well as new tools useful in controlling cellular activity.