ODOR-INDUCED PHOSPHORYLATION OF OLFACTORY CILIA PROTEINS
ODOR-INDUCED PHOSPHORYLATION OF OLFACTORY CILIA PROTEINS
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DOI：
10.1073/pnas.89.24.11983
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发表时间：
1992-12-15
影响因子：
11.1
通讯作者：
BREER, H
中科院分区：
文献类型：
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作者：
BOEKHOFF, I;SCHLEICHER, S;BREER, H
Stimulation of isolated rat olfactory cilia in the presence of [gamma-P-32]ATP leads to a significantly enhanced incorporation of [P-32]phosphate. Depending on the type of odorants applied, the induced phosphorylation is completely blocked by specific inhibitors of either protein kinase A or protein kinase C. Time-course experiments indicate that the odor-induced modification of ciliary proteins is transient; the intensity of labeling decayed over time (1-10 sec). Separation of ciliary proteins by SDS/polyacrylamide gel electrophoresis followed by autoradiography demonstrated that upon stimulation with lilial, a single polypeptide (50,000 Da) was phosphorylated; the size of the modified protein is in line with the hypothesis that odorant receptors are phosphorylated subsequent to activation by specific odors.
