Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction
Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction
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辅助生殖中与 IVF 和救援 ICSI 妊娠相关的精浆蛋白质组变化的特征
DOI：
10.1111/andr.12687
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发表时间：
2019-07-31
期刊：
影响因子：
4.5
通讯作者：
Liu, F.
中科院分区：
文献类型：
--
作者：
Liu, X.;Liu, G.;Liu, F.
背景精浆是一种很有前途的男性不育诊断液。在辅助生殖中，在体外受精失败后，通过抢救性胞浆内单精子注射获得临床妊娠成功的正常精子症男性的精浆特征仍不清楚。目的鉴定可能的精浆蛋白，以期对不明原因男性不育有新的认识。材料与方法采用等压标记相对和绝对定量标记结合高效液相色谱-基质辅助激光解吸电离电离质谱法，研究了卵胞浆内单精子注射妊娠组与体外受精组精浆中蛋白质的差异表达。结果(S)本工作揭示了73个精浆蛋白在体外受精和解救性卵胞浆内单精子注射组之间的差异表达。与体外受精组相比，卵胞浆内单精子显微注射组有45个蛋白质表达上调，28个蛋白质表达下调。生物信息学分析表明，这些改变的蛋白质参与了多种功能，包括激肽释放酶相关的蛋白水解级联反应、免疫反应和肝素结合。此外，通过蛋白质(乳铁蛋白[LTF]、纤维连接蛋白[FN1]、肌酸激酶B型[CKB]、激肽释放酶2[KLK2]、氨基肽酶N[ANPEP]、细胞外基质蛋白1[ECM1]、糖蛋白[PAEP]、α-1抗胰蛋白酶[SERPINA1]和精胶蛋白-1[SEMG1])和免疫荧光分析，验证了蛋白质组学结果的有效性。此外，目前工作中鉴定的精浆蛋白中有16%在以前的研究中没有报道。探讨不明原因男性因素不育症患者精浆蛋白的改变可能具有临床意义，对体外受精中特发性不育症的诊断和预后有一定的参考价值。结论我们的工作不仅提供了一个新的补充的高置信度精浆蛋白质数据集，而且为了解不同辅助生殖结局的正常精子症男性精浆的分子特征提供了新的线索。
Background Seminal plasma is a promising diagnostic fluid for male infertility. In assisted reproduction, the seminal plasma-based characteristics of normozoospermic men achieving successful clinical pregnancy through rescue intracytoplasmic sperm injection after in vitro fertilization failure remain unclear. Objective To identify potential seminal plasma proteins to contribute to a new understanding of unexplained male factor infertility. Materials and Methods An approach with isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography matrix-assisted laser desorption ionization mass spectrometry was applied to investigate differentially expressed proteins in the seminal plasma of a rescue intracytoplasmic sperm injection pregnancy group versus an in vitro fertilization pregnancy group of normozoospermic men. Result(s) The present work revealed seventy-three differentially expressed seminal plasma proteins between the in vitro fertilization and rescue intracytoplasmic sperm injection groups. Forty-five proteins were upregulated, and 28 proteins were downregulated in the rescue intracytoplasmic sperm injection group compared with the in vitro fertilization group. Bioinformatics analyses showed that these altered proteins were involved in various functions, including the kallikrein-related proteolytic cascade, immune response, and heparin binding. Furthermore, the validity of the proteomic results was verified by Western blot analysis of the proteins (lactoferrin [LTF], fibronectin [FN1], creatine kinase B type [CKB], kallikrein-2 [KLK2], aminopeptidase N [ANPEP], extracellular matrix protein 1 [ECM1], glycodelin [PAEP], alpha-1-antitrypsin [SERPINA1], and semenogelin-1 [SEMG1]) and immunofluorescence. Moreover, 16% of the seminal plasma proteins identified in the present work have not been reported in previous studies. Discussion This panel of altered seminal plasma proteins associated with unexplained male factor infertility might have clinical relevance and may be useful in the diagnosis and prognosis of idiopathic infertility in in vitro fertilization. Conclusions Our work not only provides a new complementary high-confidence dataset of seminal plasma proteins but also shines new light onto the molecular characteristics of seminal plasma from normozoospermic men with different assisted reproductive outcomes.