Histological investigation of human glaucomatous eyes: Extracellular fibrotic changes and galectin 3 expression in the trabecular meshwork and optic nerve head
Histological investigation of human glaucomatous eyes: Extracellular fibrotic changes and galectin 3 expression in the trabecular meshwork and optic nerve head
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DOI：
10.1002/ca.23263
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发表时间：
2018-10-01
期刊：
影响因子：
2.4
通讯作者：
Clark, Abbot F.
中科院分区：
文献类型：
--
作者：
Belmares, Ricardo;Raychaudhuri, Urmimala;Clark, Abbot F.
青光眼是导致不可逆性视力丧失的主要原因，并与两个眼组织--视神经头(ONH)和小梁网(TM)的纤维化改变有关。我们研究了青光眼患者眼内细胞外基质成分(ECM)的差异，包括胶原、弹性蛋白、转化生长因子β2、II型受体和Galectin3(Gal3)，以量化ONH和TM的纤维化变化。制备青光眼和对照组供眼，进行化学和免疫染色，以定量分析TM和ONH中ECM蛋白的表达。化学染色包括：三色(胶原)、Vernhoff-Van Giesen(弹性蛋白)和天狼星红(胶原)。免疫组织化学方法检测GAL3和转化生长因子β2RII的表达。用Image J软件进行定量分析。学生t检验用于组间比较，皮尔逊检验用于确定相关性，P值0.05(或更小)被认为具有统计学意义。青光眼组织中ECM蛋白沉积增加。青光眼TM中胶原(P=0.0469)、半乳糖3(P<；0.0001)和转化生长因子β2RⅡ(P=0.0005)均升高。在ONH青光眼中，胶原(P=0.0517)和Galectin 3(P=0.041)也明显增加。在TM(P<；0.0001)和视神经(P=0.0003)中，转化生长因子βRII与Gal3呈正相关。青光眼的TM和ONH显示ECM蛋白表达增加，支持纤维化病理。Galectin3和转化生长因子β-2R II在TM和视神经中呈正相关，支持共定位，提示它们在青光眼纤维化过程中的潜在作用。克莱恩。阿纳特。2018年1031-1049。(C)2018年作者。《临床解剖学》由威利期刊公司代表美国临床解剖学家协会出版。
Glaucoma is a leading cause of irreversible vision loss and is associated with fibrotic changes in two ocular tissues-the optic nerve head (ONH) and trabecular meshwork (TM). We investigated the differences in extracellular matrix components (ECM) including collagen, elastin, transforming growth factor beta-2, type-II receptor (TGF beta RII) and Galectin3 (Gal3) in the glaucomatous human eyes to quantify fibrotic changes in ONH and TM. Glaucomatous and control human donor eyes were prepared for chemical and immunological staining to quantify ECM protein expression in the TM and ONH. Chemical staining included: Trichrome (collagen), Vernhoeff-Van Giesen (elastin) and Sirius Red (collagen). Immunohistochemistry was used to determine levels of Gal3 and TGF beta 2RII. Quantitative analyses were performed using Image J software. Student's t-test was used to compare groups and Pearson's test was used to determine correlations P-values of 0.05 (or less) were considered statistically significant. Deposition of ECM proteins was elevated in glaucomatous tissues. There was increased collagen (P = 0.0469), Gal3 (P < 0.0001) and TGF beta 2RII (P = 0.0005) in the TM of glaucomatous eyes. Likewise, collagen (P = 0.0517) and Galectin3 (P = 0.041) were increased in the ONH glaucomatous eyes. There was a correlation of TGF beta RII with Gal3 in the TM (P < 0.0001) and optic nerve (P = 0.0003). The TM and ONH of glaucomatous eyes showed increased expression of ECM proteins supporting a fibrotic pathology. Galectin3 and TGF beta-2R II showed a positive correlation in TM and optic nerve supporting co-localization and suggesting their potential role in the glaucoma fibrotic process. Clin. Anat. 31:1031-1049, 2018. (c) 2018 The Authors. Clinical Anatomy published by Wiley Periodicals, Inc. on behalf of American Association of Clinical Anatomists.