CCR9 AND CCR7 ARE OVEREXPRESSED IN CD4 – CD8 – THYMOCYTES OF MYASTHENIA GRAVIS PATIENTS
CCR9 AND CCR7 ARE OVEREXPRESSED IN CD4 – CD8 – THYMOCYTES OF MYASTHENIA GRAVIS PATIENTS
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CCR9 和 CCR7 在重症肌无力患者的 CD4 – CD8 – 胸腺细胞中过度表达
DOI：
10.1002/mus.24999
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
YING DU
中科院分区：
文献类型：
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作者：
QIANRU LI;PINGPING LIU;XIAOYAN XUAN;JUNFENG ZHANG;YUN ZHANG;ZHENGKUN ZHU;FENG GAO;QINGYONG ZHANG;YING DU
简介：趋化因子CC基序受体9和7（CCR 9和CCR 7）在T细胞前体迁移到胸腺以启动T胸腺生成中起主要作用。方法：采用流式细胞术和免疫荧光法检测CCR 9+和CCR 7+细胞在重症肌无力（MG）患者外周血T细胞中的表达和分布。结果：与非MG胸腺相比，MG胸腺中DN胸腺细胞CCR 9、CCR 7表达增加，CCR 9、CCR 7、IL-7 R mRNA水平升高，CXCR 4水平降低。在MG胸腺中，更多的CCR 7和CCR 9双阳性（DP）胸腺细胞聚集在被膜下区域附近。结论：CCR 9和CCR 7的表达增强可能使MG胸腺中DP胸腺细胞从DN期分化复杂化。肌肉神经，2016肌肉神经55：84-90，2017
Introduction: Chemokine CC motif receptors 9 and 7 (CCR9 and CCR7) play a major role in the migration of T‐cell precursors to the thymus to initiate T thymopoiesis. However, their role in development of T‐cells in myasthenia gravis (MG) patients has not been fully elucidated.Methods: Expression and distribution of CCR9+and CCR7+cells were detected by flow cytometry and immunofluorescence. Real‐time polymerase chain reaction was used to check the adhesion molecules on CD4–CD8–double‐negative (DN) thymocytes.Results: CCR9 and CCR7 expression by DN thymocytes increased in the MG thymus; the levels of CCR9, CCR7, interleukin‐7R mRNA increased, and CXCR4 levels decreased compared with levels in the non‐MG thymus. More CCR7 and CCR9 double‐positive (DP) thymocytes were gathered near the subcapsular region in MG thymus.Conclusions: Enhanced expression of CCR9 and CCR7 may complicate the differentiation of DP thymocytes from the DN stage in MG thymus.Muscle Nerve, 2016Muscle Nerve55: 84–90, 2017