Phototoxic process after rapid photosensitive membrane damage of 5,5"-bis(aminomethyl)-2,2':5',2"-terthiophene dihydrochloride.
Phototoxic process after rapid photosensitive membrane damage of 5,5"-bis(aminomethyl)-2,2':5',2"-terthiophene dihydrochloride.
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5,5"-双(氨基甲基)-2,2:5,2"-三联噻吩二盐酸盐快速光敏膜损伤后的光毒性过程。
DOI：
10.1016/s1011-1344(01)00167-1
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Kunihiko Mabuchi
中科院分区：
文献类型：
--
作者：
Takashi K. Saito;Mareyuki Takahashi;Hitoshi Muguruma;Etsuo Niki;Kunihiko Mabuchi
我们报道了一个新的方面的快速（<30 s）光诱导的细胞膜损伤光敏5，5“-双（氨甲基）-2，2”：5“，2”-三噻吩二盐酸盐（BAT），这是一种水溶性的α-三噻吩类似物，使用高功率激光（光强度1.6 W cm−2）。本文将讨论细胞在光敏剂作用下的时间与光毒性过程之间的关系。可以确定三种毒性过程：第一，依赖于BAT细胞孵育的非光介导的毒性;第二，当BAT浓度在2-10 μM范围内时，不依赖于BAT暴露时间的光毒性;第三，当BAT浓度达到20 μM时，依赖于BAT暴露时间的光毒性。当α-生育酚（一种抗氧化剂）加入细胞膜时，细胞毒性降低。这种光毒性模式是由BAT三重态产生的活性氧引发的磷脂过氧化链式反应和膜蛋白氧化损伤的典型模式。BAT暴露时间与光敏剂在细胞膜和细胞内的分配明显相关。
We report a new aspect of rapid (<30 s) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue, using a high-power laser (light intensity 1.6 W cm−2). In this paper, we will discuss the relationship between the exposure time of the cells to the photosensitizer and the phototoxic process. Three toxic processes can be identified: first, a non-light-mediated toxicity dependent on BAT-cell incubation; second, a phototoxicity independent of BAT exposure time when the BAT concentration is in the 2–10-μM range; third, a phototoxicity dependent on BAT exposure time when BAT concentration becomes 20 μM. The cytotoxicity decreases when α-tocopherol, an antioxidant, is added to a cell membrane. This pattern of phototoxicity is the typical of a phospholipid peroxidation chain reaction and oxidative damage of membrane proteins triggered by a reactive oxygen species generated by a triplet state of BAT. The BAT exposure time is clearly correlated with the partition of the photosensitizer in the cell membrane and inside the cell.
光诱导细胞质膜损伤的动力学。
影响因子：
3.4
作者：
Thorpe,WP;Toner,M;Ezzell,RM;Tompkins,RG;Yarmush,ML
通讯作者：
Yarmush,ML