12-O-tetradecanoylphorbol-13-acetate activates phosphatidylethanol and phosphatidylglycerol synthesis by phospholipase D in cell lysates.
12-O-tetradecanoylphorbol-13-acetate activates phosphatidylethanol and phosphatidylglycerol synthesis by phospholipase D in cell lysates.
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12-O-tetradecanoylphorbol-13-acetate 通过细胞裂解物中的磷脂酶 D 激活磷脂酰乙醇和磷脂酰甘油的合成。
DOI：
10.1016/s0006-291x(88)81076-3
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发表时间：
1988
影响因子：
3.1
通讯作者：
Mueller,GC
中科院分区：
文献类型：
--
作者：
Tettenborn,CS;Mueller,GC
在 HL-60 细胞超声处理中开发了用于合成磷脂酰醇的无细胞系统。以[32P]磷脂酰胆碱为外源底物，通过磷脂酶D催化乙醇和甘油的转磷脂酰化分别形成磷脂酰乙醇和磷脂酰甘油。磷脂酶 D 的转磷脂酰化作用在体外由 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 刺激，并且需要添加 ATP 才能获得最佳反应。 GTP-γ-S（G 蛋白系统的激活剂）也通过独立机制刺激该过程。据推测，通过磷脂酶 D 激活刺激磷脂代谢代表了一种重要机制，TPA 可能通过改变脂质环境来调节细胞内信号生成系统或影响膜结合蛋白的活性。
A cell-free system for the synthesis of phosphatidylalcohols was developed in sonicates of HL-60 cells. With [32P]phosphatidylcholine as the exogenous substrate, both phosphatidylethanol and phosphatidylglycerol were formed through a phospholipase D-catalyzed transphosphatidylation of ethanol and glycerol, respectively. The transphosphatidylation by phospholipase D was stimulatedin vitroby 12-O-tetradecanoylphorbol-13-acetate (TPA) and required the addition of ATP for an optimal response. GTP-γ-S, an activator of G protein systems, also stimulated the process by an independent mechanism. It is postulated that the stimulation of phospholipid metabolism through phospholipase D activation represents an important mechanism whereby TPA might modulate intracellular signal generating systems or influence the activity of membrane-bound proteins by altering their lipid environment.