Concentration of Dissolved Amino Acids from Saline Waters by Ligand-Exchange Chromatography
Concentration of Dissolved Amino Acids from Saline Waters by Ligand-Exchange Chromatography
复制标题
通过配体交换色谱法浓缩盐水中的溶解氨基酸
DOI：
10.1126/science.151.3714.1098
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发表时间：
1966
期刊：
影响因子：
56.9
通讯作者：
E. Degens
中科院分区：
文献类型：
--
作者：
A. Siegel;E. Degens
溶解在盐溶液中的氨基酸可以通过铜- chelex 100树脂的配体交换来浓缩并从溶液中去除。竞争的无机配体不会干扰，离子与阳离子的交换也不会发生；这样就避免了金属离子从该柱中流失。为了测试配体交换色谱的潜力，研究了海水中溶解的氨基化合物的类型和性质。数据显示，大部分溶解的氨基化合物以结合状态存在，而不是自由状态。
Amino acids dissolved in salt solutions may be concentrated and removed from the solution by ligand exchange on copper-Chelex 100 resin. Competing inorganic ligands do not interfere, and ion exchange with cations does not occur; thus loss of metal ion from this column is avoided. To test the potentiality of ligand exchange for chromatography, the type and nature of the dissolved amino compounds in sea water were investigated. The data revealed that the bulk of the dissolved amino compounds is present in a combined rather than a free state.