EFFECT OF 1,25-DIHYDROXYVITAMIN D3 ON INTESTINAL CALCIUM-ABSORPTION IN STRONTIUM-FED RATS
EFFECT OF 1,25-DIHYDROXYVITAMIN D3 ON INTESTINAL CALCIUM-ABSORPTION IN STRONTIUM-FED RATS
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DOI：
10.1016/0003-9861(79)90116-4
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发表时间：
1979-01-01
影响因子：
3.9
通讯作者：
BRUNS, MEH
中科院分区：
文献类型：
--
作者：
ARMBRECHT, HJ;WASSERMAN, RH;BRUNS, MEH
本文研究了1，25-二羟维生素D_3对大鼠小肠钙吸收的初始刺激作用。为了产生功能性维生素D3缺乏症，大鼠喂食含有2.4%锶的饮食。在饮食10天后，肠钙吸收，通过外翻肠囊测量，显着抑制。锶喂养的大鼠口服1，25-二羟维生素D3，并在肠道钙摄取，肠碱性磷酸酶活性，肠钙结合蛋白的变化进行了测量剂量后的时间函数。4 h时，十二指肠近端2.5 cm处的钙摄取显著增加，7 h时沿着整个十二指肠的钙摄取显著增加。肠碱性磷酸酶活性，在Triton提取物的粘膜匀浆和分离刷状缘复合物中测定，也增加了7小时。使用凝胶电泳和免疫扩散对一个特定的抗血清，增加肠钙结合蛋白，检测到肠上清液在给药后4小时。几乎没有钙结合蛋白是可检测到的锶喂养大鼠与丙二醇。这些时间研究与碱性磷酸酶和钙结合蛋白在1，25-二羟维生素D3刺激的肠道钙摄取中的作用一致。此外，锶喂养的有用性，在大鼠中产生功能性维生素D3缺乏症被证明。
The initial stimulation of intestinal Ca absorption in the rate by 1,25-dihydroxyvitamin D3 was studied. To produce a functional vitamin D3-deficiency, rats were fed a diet containing 2.4% Sr. After 10 days on the diet, intestinal Ca uptake, as measured by everted gut sacs, was significantly depressed. Sr-fed rats were dosed orally with 1,25-dihydroxyvitamin D3, and changes in intestinal Ca uptake, intestinal alkaline phosphatase activity, and intestinal Ca-binding protein were measured as a function of time after dose. Ca uptake was significantly increased in the proximal 2.5 cm of the duodenum at 4 h and along the whole duodenum by 7 h. Intestinal alkaline phosphatase activity, measured in a Triton extract of the mucosal homogenate and in isolated brush border complexes, was also increased by 7 h. Using both gel electrophoresis and immunodiffusion against a specific antiserum, an increase in intestinal Ca-binding protein was detected in intestinal supernate at 4 h after dosing. Almost no Ca-binding protein was detectable in Sr-fed rats dosed with propylene glycol only. These time studies are consistent with a role for both alkaline phosphatase and Ca-binding protein in the 1,25-dihydroxyvitamin D3-stimulated uptake of Ca by the intestine. In addition, the usefulness of Sr feeding for producing a functional vitamin D3 deficiency in rats was demonstrated.