Environmental co-regulation of substance P, somatostatin and neurotransmitter synthesizing enzymes in cultured sympathetic neurons
Environmental co-regulation of substance P, somatostatin and neurotransmitter synthesizing enzymes in cultured sympathetic neurons
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培养交感神经元中P物质、生长抑素和神经递质合成酶的环境协同调节
DOI：
10.1016/0006-8993(84)90693-0
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
J. Kessler
中科院分区：
文献类型：
--
作者：
J. Kessler
在新生大鼠颈上神经节（SCG）分离细胞培养中，研究了调节肽神经递质代谢的机制。松果体是SCG的靶点，它产生一种可溶性因子（PCM），在有神经节非神经元细胞存在的交感神经元中，使P物质（SP）水平增加15倍以上。非神经元细胞的消除使SP降低到可忽略不计的水平，并消除了PCM对SP表达的刺激作用。这些观察结果表明，神经节非神经元细胞刺激SP的交感表达，松果体通过作用于神经节支持细胞或与之协同作用来影响神经元SP。PCM还影响其他神经递质系统。在神经节非神经元细胞存在的情况下，PCM处理增加了胆碱乙酰转移酶（CHAC），降低了酪氨酸羟化酶（TOH）和生长抑素（SO）。相比之下，PCM处理纯神经元培养物导致echac和SP水平可忽略不计，SO增加一倍，TOH小幅增加。在交感神经元中，SP表达可能与胆碱能发育有关，而SO可能与去肾上腺素能表型表达有关。此外，SP和SS在交感神经元中的表达与之前描述的去甲肾上腺素能和胆碱能表达之间的关系类似17 - 19。
Mechanisms regulating peptide neurotransmitter metabolism were examined in dissociated cell cultures of the neonatal rat superior cervical ganglion (SCG). The pineal gland, a target of the SCG, produced a soluble factor (PCM) which increased substance P (SP) levels more than 15-fold in sympathetic neurons cultured in the presence of ganglion non-neuronal cells. Elimination of the non-neuronal cells decreased SP to negligible levels and abolished the stimulatory effects of PCM on SP expression. These observations suggest that ganglion non-neuronal cells stimulate sympathetic expression of SP, and that the pineal influences neuronal SP by acting on, or in concert with, ganglion support cells. PCM also influenced other neurotransmitter systems. In the presence of ganglion non-neuronal cells, PCM treatment increased cholineacetyltransferase (CHAC) and decreased tyrosine hydroxylase (TOH) and somatostatin (SO). By contrast, PCM treatment of pure neuronal cultures resulted in negligibleCHAC and SP levels and a doubling of SO with a small increase in TOH. In sympathetic neurons, SP expression may be associated with cholinergic development, whereas SO may be associated with noradrenergic phenotypic expression. Moreover, there is a reciprocal relationship between SP and SS expression by sympathetic neurons analogous toe previously described relationship between noradrenergic and cholinergic expression17–19.
培养物中交感神经元 P 物质的靶器官调节。
影响因子：
2.7
作者：
Kessler,JA;Adler,JE;Jonakait,GM;Black,IB
通讯作者：
Black,IB