Analysis of macromolecular ethylene oxide adducts
Analysis of macromolecular ethylene oxide adducts
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大分子环氧乙烷加合物的分析
DOI：
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发表时间：
1988
影响因子：
3
通讯作者：
U. Föst
中科院分区：
文献类型：
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作者：
H. Bolt;H. Peter;U. Föst
环氧乙烷是一种致癌化合物，也是乙烯的代谢产物。环氧乙烷与蛋白质和核酸形成大分子加合物。蛋白质中的靶标是氨基酸半胱氨酸、组氨酸和缬氨酸（如果是N末端，如血红蛋白中）。主要的DNA加合物是7-（2-羟乙基）-鸟嘌呤。用于检测这种加合物的方法包括放射性标记和GC-MS。通过选择性富集DNA样品中的加合物，可以提高当前GC/MS方法的灵敏度。目前正在进行这方面的研究。
SummaryEthylene oxide is a carcinogenic compound which is also an ethylene metabolite. Ethylene oxide forms macromulecular adducts with proteins and nucleic acids. Targets in proteins are the amino acids cysteine, histidine and valine (if N-terminal, as in hemoglobin). The major DNA adduct is 7-(2-hydroxyethyl)-guanine. Methods for detection of this adduct include radiolabelling and GC-MS. The sensitivity of current GC/MS methods can be improved by selective enrichment of adducts from DNA samples. Studies in this direction are presently being performed.