An arabinogalactan from fruits of Lycium barbarum L. inhibits production and aggregation of Aβ42.
An arabinogalactan from fruits of Lycium barbarum L. inhibits production and aggregation of Aβ42.
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DOI：
10.1016/j.carbpol.2018.05.022
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发表时间：
2018-09
影响因子：
11.2
通讯作者：
Lishuang Zhou;Wenfeng Liao;Xia Chen;Hang Yue;Saijuan Li;K. Ding
中科院分区：
文献类型：
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作者：
Lishuang Zhou;Wenfeng Liao;Xia Chen;Hang Yue;Saijuan Li;K. Ding
β淀粉样蛋白（Aβ）诱导的神经变性被认为是阿尔茨海默病（AD）的病理机制之一。抑制 Aβ 产生或聚集是抗 AD 药物发现的有前途的治疗策略之一。本研究从枸杞果实中纯化得到平均分子量为45.0kDa的均质中性多糖，命名为LBP1A1-1。其结构特征为具有1, 3-连接的β-Galp、1, 6-连接的β-Galp、1, 4-连接的α-Glcp的主链，并在1, 6-连接的β-Galp的C-3位置处被1, 3-连接的β-Galp的C-6位置取代。分支包含末端(T)-连接的β-Galp、T-连接的α-Araf、T-连接的β-Araf、1, 5-连接的α-Araf和T-连接的β-Rhap。体外实验表明，LBP1A1-1能够以剂量依赖的方式抑制Aβ42的产生并阻碍Aβ42的聚集，且无细胞毒性。这些结果表明LBP1A1-1可能具有治疗AD的多重潜力。
Theβamyloid (Aβ) induced neurodegeneration is believed to be one of pathological mechanisms of Alzheimer’s disease (AD). The inhibition of Aβproduction or aggregation is one of the promising therapeutic strategies for anti-AD drug discovery. Here, a homogeneous neutral polysaccharide designated LBP1A1-1 with an average molecular weight of 45.0 kDa was purified from fruits ofLycium barbarumL. Its structure was characterized to possess a backbone of 1, 3-linked β-Galp, 1, 6-linked β-Galp, 1, 4-linked α-Glcpwith branches substituted at C-3 position of 1, 6-linked β-Galpor C-6 position of 1, 3-linked β-Galp. The branches contained terminal (T)-linked β-Galp, T-linked α-Araf, T-linked β-Araf, 1, 5-linked α-Arafand T-linked β-Rhap. Thein vitroexperiments revealed that LBP1A1-1 could inhibit Aβ42production and impede Aβ42aggregation in a dose-dependent-manner without cytotoxicity. These results suggested that LBP1A1-1 might have the multiple potential for the treatment of AD.