Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene.
Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene.
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第二个果蝇蛋白激酶 A 催化亚基基因的活性、表达和功能。
DOI：
10.1093/genetics/141.4.1507
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发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
Kalderon,D
中科院分区：
文献类型：
--
作者：
Meléndez,A;Li,W;Kalderon,D
DC2基因与果蝇蛋白激酶A （PKA）的主要催化亚基基因DC0序列相似，此前已被分离出来。我们在这里展示了67-kD果蝇DC2蛋白在体外表现为PKA催化亚基。DC2在早期胚胎的中胚层胶原中转录。这种表达依赖于背部，但既不依赖于扭转也不依赖于蜗牛的活动。DC2转录融合物模拟了这种胚胎表达，也在三龄幼虫的视膜、翅盘和腿盘的细胞亚群中表达。对于隐性致死突变，对含有DC2的小缺陷间隔进行饱和筛选，未发现DC2等位基因。因此，我们分离出新的缺陷以产生缺乏DC2活性的缺陷反杂合子。这些动物都是能繁殖的。DC2的缺失不影响缺乏DC0活性的影像学椎间盘细胞的活力或表型，也不影响DC0活性降低的动物的胚胎孵化。此外，从DC0启动子表达DC2的转基因并不能有效地挽救多种DC0突变表型。这些观察结果表明，DC2不是必需基因，不太可能与DC0在功能上冗余，DC0在发育过程中具有多种独特的功能。
The DC2 gene was isolated previously on the basis of sequence similarity to DC0, the major Drosophila protein kinase A (PKA) catalytic subunit gene. We show here that the 67-kD Drosophila DC2 protein behaves as a PKA catalytic subunit in vitro. DC2 is transcribed in mesodermal anlagen of early embryos. This expression depends on dorsal but on neither twist nor snail activity. DC2 transcriptional fusions mimic this embryonic expression and are also expressed in subsets of cells in the optic lamina, wing disc and leg discs of third instar larvae. A saturation screen of a small deficiency interval containing DC2 for recessive lethal mutations yielded no DC2 alleles. We therefore isolated new deficiencies to generate deficiency trans-heterozygotes that lacked DC2 activity. These animals were viable and fertile. The absence of DC2 did not affect the viability or phenotype of imaginal disc cells lacking DC0 activity or embryonic hatching of animals with reduced DC0 activity. Furthermore, transgenes expressing DC2 from a DC0 promoter did not efficiently rescue a variety of DC0 mutant phenotypes. These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple unique functions during development.