Novel Husbandry Practices Result in Rapid Rates of Growth and Sexual Maturation Without Impacting Adult Behavior in the Blind Mexican Cavefish.
Novel Husbandry Practices Result in Rapid Rates of Growth and Sexual Maturation Without Impacting Adult Behavior in the Blind Mexican Cavefish.
复制标题
新的饲养方法可以使墨西哥盲眼洞穴鱼快速生长和性成熟，而不影响成年行为。
DOI：
10.1089/zeb.2023.0001
复制
发表时间：
2023
期刊：
影响因子：
2
通讯作者：
Duboué,ErikR
中科院分区：
文献类型：
--
作者：
Kozol,RobertA;Yuiska,Anders;Han,JiHeon;Tolentino,Bernadeth;Lopatto,Arthur;Lewis,Peter;Paz,Alexandra;Keene,AlexC;Kowalko,JohannaE;Duboué,ErikR
动物模型系统依赖于最大化生长和减少世代时间的饲养协议的标准化。墨西哥四，Astyanax mexicanus，存在于有眼睛的表面和盲目的洞穴居住人口。在独立进化的种群之间进行比较的机会导致了A.墨西哥是进化和生物医学研究的典范。然而，缓慢和不稳定的生长速度仍然是扩大应用的主要限制。墨西哥人幸运的是，这种时间限制可以通过畜牧业的变化来解决，在保持最佳健康结果的同时加快增长率。在这里，我们描述了一个畜牧协议，通过改变饮食，喂养频率，生长分选和逐步改变坦克大小产生快速增长率。与我们以前的方案相比，该方案产生了稳健的生长率并降低了性成熟年龄。为了确定喂养的变化是否会影响行为，我们在探索和学校试验中测试了鱼类。我们发现两组之间的行为没有差异，这表明增加进食和快速生长不会影响行为特征的自然变化。因此，该标准化畜牧规程的制定将促进我国畜牧业的发展.墨西哥是一个遗传模型。
Animal model systems are dependent on the standardization of husbandry protocols that maximize growth and reduce generation time. The Mexican tetra,Astyanax mexicanus, exists as eyed surface and blind cave dwelling populations. The opportunity for comparative approaches between independently evolved populations has led to the rapid growth ofA. mexicanusas a model for evolution and biomedical research. However, a slow and inconsistent growth rate remains a major limitation to the expanded application ofA. mexicanus. Fortunately, this temporal limitation can be addressed through husbandry changes that accelerate growth rates while maintaining optimal health outcomes. Here, we describe a husbandry protocol that produces rapid growth rates through changes in diet, feeding frequency, growth sorting and progressive changes in tank size. This protocol produced robust growth rates and decreased the age of sexual maturity in comparison to our previous protocol. To determine whether changes in feeding impacted behavior, we tested fish in exploration and schooling assays. We found no difference in behavior between the two groups, suggesting that increased feeding and rapid growth will not impact the natural variation in behavioral traits. Taken together, this standardized husbandry protocol will accelerate the development ofA. mexicanusas a genetic model.