Sputnik: a database platform for comparative plant genomics
Sputnik: a database platform for comparative plant genomics
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DOI：
10.1093/nar/gkg075
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发表时间：
2003-01-01
影响因子：
14.9
通讯作者：
Mayer, KFX
中科院分区：
文献类型：
--
作者：
Rudd, S;Mewes, HW;Mayer, KFX
来自20个不同植物物种的200万个植物EST，总计超过1000 Mbp的DNA序列，代表了一个强大的转录组资源。Sputnik利用这种序列资源的潜力来填补未测序的植物基因组中的一些信息空白，并作为硅比较植物基因组学的基础。使用优化的EST聚类技术降低了单个EST集合的复杂性。簇序列的注释是通过利用和转移来自已经为完整的模型植物基因组（拟南芥）产生的综合知识库的信息，并通过进行与当今植物生物学家相关的额外的最先进的序列分析来进行的。对50万个植物EST衍生肽的功能预测、比较分析和关联注释使Sputnik（http：//mips.gsf.de/proj/Sputnik/）成为当代植物基因组学的有效平台。
Two million plant ESTs, from 20 different plant species, and totalling more than one 1000 Mbp of DNA sequence, represents a formidable transcriptomic resource. Sputnik uses the potential of this sequence resource to fill some of the information gap in the un-sequenced plant genomes and to serve as the foundation for in silicio comparative plant genomics. The complexity of the individual EST collections has been reduced using optimised EST clustering techniques. Annotation of cluster sequences is performed by exploiting and transferring information from the comprehensive knowledge-base already produced for the completed model plant genome ( Arabidopsis thaliana) and by performing additional state of-the-art sequence analyses relevant to today's plant biologist. Functional predictions, comparative analyses and associative annotations for 500 000 plant EST derived peptides make Sputnik (http://mips.gsf.de/proj/sputnik/) a valid platform for contemporary plant genomics.