Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS
Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS
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DOI：
10.1021/pr7008109
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Sanders, Melinda E.;Dias, Eduardo C.;Caprioli, Richard M.
我们使用MALDI MS和激光捕获显微切割细胞评估了乳腺癌的蛋白质组模式。比较了浸润性乳腺癌与正常乳腺上皮以及雌激素受体阳性与雌激素受体阴性肿瘤中蛋白质和肽的表达。通过统计分析鉴定生物标志物候选物，并在盲法测试集中开发和验证分类器。分类器中使用的几个m/z特征通过LC-MS/MS鉴定，两个通过免疫组织化学确认。
We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.