Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams.
Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams.
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用电喷雾液滴离子束对肾细胞癌的次级离子质谱分析。
DOI：
10.5702/massspectrometry.a0053
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hiraoka K
中科院分区：
文献类型：
--
作者：
Ninomiya S;Yoshimura K;Chen LC;Takeda S;Hiraoka K
采用电喷雾液滴离子束诱导二次离子质谱（EDI/西姆斯）对肾癌患者的组织样品进行了分析。测量组织样本的癌性和非癌性区域的带正电荷和带负电荷的二次离子。虽然在正负二次离子谱中均未发现特定的癌性物种，但癌组织和非癌组织的谱呈现不同的趋势。例如，在500-800的m/z范围内，癌组织的正二次离子谱中，几个m/z值的强度低于m/z+2峰的强度（表明该物种的一个双键损失），而对于非癌组织，获得相反的趋势。还使用探针电喷雾电离（PESI）对组织样本进行了串联质谱（MS/MS），发现PESI产生的一些分子离子被碎片化为EDI/西姆斯分析中观察到的离子。对EDI/西姆斯产生的正二次离子谱进行主成分分析时，癌组织和非癌组织的结果是分离的。EDI/西姆斯方法可以应用于以高概率区分癌性和非癌性区域。
Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in the m/z range of 500–800 of the positive secondary ion spectra for the cancerous tissues, the intensities for several m/z values were lower than those of the m/z+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability.