The Type 4 Pili of Enterohemorrhagic Escherichia coli O157:H7 Are Multipurpose Structures with Pathogenic Attributes
The Type 4 Pili of Enterohemorrhagic Escherichia coli O157:H7 Are Multipurpose Structures with Pathogenic Attributes
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DOI：
10.1128/jb.01306-08
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Giron, Jorge A.
中科院分区：
文献类型：
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作者：
Xicohtencatl-Cortes, Juan;Monteiro-Neto, Valerio;Giron, Jorge A.
肠出血性大肠杆菌（EHEC）O 157：H7产生称为HCP（出血性大肠杆菌皮利）的长束极性4型皮利（T4 P），其在与人类和动物上皮细胞相关的细菌之间形成物理桥梁。在这里，我们试图进一步研究HCP是否具有与T4P产生相关的其他致病性属性。与野生型EHEC EDL 933和同基因hcpA突变体进行的比较研究表明，HCP在这种生物体的生物学中发挥不同的作用。我们发现，除了促进细菌附着到宿主细胞，HCP介导（i）上皮细胞的侵入，（ii）兔红细胞的血凝，（iii）有利于生物膜形成的细菌间连接，（iv）与宿主细胞外基质蛋白层粘连蛋白和纤连蛋白特异性结合，但不与胶原蛋白结合，以及（v）抽搐运动。非粘附性实验室E.在质粒pJX22上与hcpABC基因互补的大肠杆菌菌株HB 101，其指定在EDL 933中HCP过量产生，变得过度粘附和侵入，并产生厚的生物膜，表明HCP的存在赋予HB 101（pJX22）新的属性，否则HB 101不会表现出。类似于T4P参与几种传染病发病机制的其他细菌，我们的数据强烈表明HCP显示多种功能，可能有助于EHEC在不同宿主中的定植，以及这种食源性病原体的毒力、存活和传播。
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 produces long bundles of polar type 4 pili (T4P) called HCP (for hemorrhagic coli pili) that form physical bridges between bacteria associating with human and animal epithelial cells. Here, we sought to further investigate whether HCP possessed other pathogenicity attributes associated with T4P production. Comparative studies performed with wild-type EHEC EDL933 and an isogenic hcpA mutant revealed that HCP play different roles in the biology of this organism. We found that in addition to promoting bacterial attachment to host cells, HCP mediate (i) invasion of epithelial cells, (ii) hemagglutination of rabbit erythrocytes, (iii) interbacterial connections conducive to biofilm formation, (iv) specific binding to host extracellular matrix proteins laminin and fibronectin but not collagen, and (v) twitching motility. Nonadherent laboratory E. coli strain HB101 complemented with hcpABC genes on plasmid pJX22, which specifies for HCP overproduction in EDL933, became hyperadherent and invasive and produced a thick biofilm, suggesting that the presence of HCP confers HB101(pJX22) new attributes otherwise not exhibited by HB101. Analogous to other bacteria in which T4P are involved in the pathogenesis of several infectious diseases, our data strongly suggest that HCP display multiple functions that may contribute to EHEC colonization of different hosts and to virulence, survival, and transmission of this food-borne pathogen.