Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips
Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips
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DOI：
10.1023/b:ticu.0000007283.28280.a3
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发表时间：
2004-02-01
影响因子：
3
通讯作者：
Nair, GM
中科院分区：
文献类型：
--
作者：
Decruse, SW;Seeni, S;Nair, GM
以濒危药用植物轮瓣全缘木（Holostemaannulare）茎尖为试材，采用MS培养基（MS-1、MS-2、MS-3）进行了茎尖超低温保存试验。在四个制备程序期间测试三种培养基组合，即：文化的发展;茎尖插条的预处理;封装茎尖的预培养;和冷冻后恢复，以了解NH 4 NO3敏感性的最关键阶段。在最初的三个制备步骤中使用的MS-1支持10.9-16.6%冷冻保存的茎尖的冷冻后回收率。在MS-1中培养和随后在MS-2或MS-3中传代（第2、3和4次制备步骤）将回收率提高至26.4- 35.8%。在整个步骤中使用的MS-3有利于38.5%的回收率。茎尖培养提出了在MS-2预处理MS-2或MS-3和随后的预培养的封装茎尖和MS-3冷冻后恢复培养的MS-2显示最大的再生（55%）。整个过程中使用的MS-2支持48%的冻存茎尖再生。
Shoot tip cryopreservation of Holostemma annulare, an endangered medicinal plant was carried out using Murashige-Skoog (MS) medium containing mM NH4++NO3-; 20.6+39.4 (MS-1), 2.6+18.8 (MS-2) or 0.0+18.8 (MS-3). The three media combinations were tested during four preparative procedures viz.: development of cultures; preconditioning of shoot tip cuttings; preculture of encapsulated shoot tips; and post-freeze recovery to understand the most critical phase of NH4NO3 sensitivity. MS-1 used during the initial three preparative steps supported 10.9-16.6% post-freeze recovery of cryopreserved shoot tips. Development of culture in MS-1 and subsequent passages (2nd, 3rd and 4th preparative steps) in MS-2 or MS-3 improved the recovery rate to 26.4-35.8%. MS-3 used throughout the steps favoured 38.5% recovery. Shoot tips from shoot cultures raised in MS-2 upon preconditioning in MS-2 or MS-3 and subsequent preculture of encapsulated shoot tips and post-freeze recovery culture in MS-3 showed maximum regeneration (55%). MS-2 used throughout the procedure supported 48% regeneration of cryopreserved shoot tips.