Beta-galactosidase and alpha-mannosidase inhibit formation of multicellular nodules in breast cancer cell cultures.
Beta-galactosidase and alpha-mannosidase inhibit formation of multicellular nodules in breast cancer cell cultures.
复制标题
DOI：
--
复制
发表时间：
2004
影响因子：
2
通讯作者：
K. Arcaro;Jo C. Wang;C. Otis;B. Zuckerman
中科院分区：
文献类型：
--
作者：
K. Arcaro;Jo C. Wang;C. Otis;B. Zuckerman
在雌激素作用下，MCF - 7细胞培养的融合单层会形成多细胞结节，称为病灶。在生理水平的17β - 雌二醇作用下，融合后病灶会形成，并且可被通过雌激素受体或芳香烃受体起作用的各种抗雌激素抑制。在本文中，我们报道膜受体末端糖类的破坏会导致病灶形成受抑制。用0.013 - 0.05单位/毫升的β - 半乳糖苷酶处理完全抑制了病灶的形成，同时细胞单层保持完整。用α - 甘露糖苷酶进行的试验导致了类似但效力较弱的病灶抑制。凝集素 - 荧光偶联物、蓖麻凝集素（RCA）和伴刀豆凝集素A（ConA）被用于鉴定MCF - 7细胞的膜表面碳水化合物。蓖麻凝集素 - 荧光偶联物的结合可被半乳糖或乳糖共同处理所抑制。伴刀豆凝集素A - 荧光偶联物的结合可被甘露糖和N - 乙酰 - 葡萄糖胺显著抑制。这是关于通过破坏膜受体表面碳水化合物来抑制MCF - 7细胞培养中病灶形成的首次报道。
In response to an estrogen, confluent monolayers of MCF-7 cell cultures develop multi-cellular nodules, termed foci. Post-confluent development of foci occurs with physiologic levels of 17beta-estradiol and are inhibited by various anti-estrogens acting through either the estrogen or aryl hydrocarbon receptors. In the present paper we report that disruption of the terminal sugars on membrane receptors results in inhibition of foci. Treatment with 0.013-0.05 units/ml of beta-galactosidase completely inhibited the development of foci while leaving the monolayer of cells intact. Trials with alpha-mannosidase resulted in a similar but less potent inhibition of foci. Lectin-fluorescent conjugates, RCA (Ricinus communis agglutinin), and ConA (Canavalia ensiformis agglutinin) were used to identify membrane surface carbohydrates on MCF-7 cells. Binding of the RCA-fluorescent conjugate was inhibited by co-treatment with galactose or lactose. Binding of ConA-fluorescent conjugate was significantly inhibited by mannose and n-acetyl-glucosamine. This is the first report of inhibition of foci development in MCF-7 cell cultures by disruption of surface carbohydrates on membrane receptors.