Ectodermal-neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β-catenin pathway
Ectodermal-neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β-catenin pathway
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外胚层神经皮质 1 作为一种新型生物标志物，通过促进 Wnt/β-catenin 通路预测乳腺癌的不良预后并诱导转移
DOI：
10.1111/jcmm.15520
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发表时间：
2020-07-03
影响因子：
5.3
通讯作者：
He, Jianjun
中科院分区：
文献类型：
--
作者：
Zhou, Yuhui;Tang, Xiaojiang;He, Jianjun
乳腺癌作为最常见的恶性肿瘤，是妇女癌症相关死亡的第二大原因。ketch家族成员之一ENC 1参与各种病理生理过程。但ENC 1在乳腺癌中的作用尚未研究。本研究对ENC 1在乳腺癌中的表达特点、临床意义及其分子机制进行了探讨。在癌症基因组图谱数据库和人类样本中研究乳腺癌和正常乳腺组织中ENC 1的表达和预后价值。敲低ENC 1以探索其在各种乳腺癌细胞系中的功能。采用Western blot方法探讨其可能的分子机制。我们观察到ENC 1在乳腺癌组织中过表达。在乳腺癌患者中，ENC 1过表达与高转移相关，并预测预后不良。ENC 1敲低抑制乳腺癌细胞的生长、克隆形成、迁移和侵袭。机制分析显示ENC 1通过调节β-catenin通路与肿瘤转移密切相关。我们的研究强调ENC 1是一个潜在的乳腺癌预后和转移相关的标志物，并可能作为一个可能的治疗靶点。
Breast cancer, as the most common malignancy, is the second leading cause of cancer-related death in women. One of the ketch family member ENC1 is involved in various pathophysiologic processes. But the role of ENC1 in breast cancer has not been investigated. The present study value the feature, clinical significance and the molecular mechanisms of ENC1 in breast cancer. The expression and prognosis value of ENC1 expression among breast cancer and normal breast tissue were investigated in The Cancer Genome Atlas database and human samples. ENC1 was knockdown to explore its function in various breast cancer cell lines. Western blot was performed to explore the potential molecular mechanisms. We observed that ENC1 was overexpressed in breast cancer tissues. ENC1 overexpression was associated with high metastasis and predicted a poor prognosis in patients with breast cancer. ENC1 Knockdown inhibits the growth, clone formation, migration and invasion of breast cancer cells. Mechanism analysis revealed ENC1 was strong associated with the metastasis by modulating beta-catenin pathway. Our study emphasizes that ENC1 is a potential prognostic and metastasis-related marker of breast cancer, and may function as a possible therapeutic target against breast cancer.