Differential neuronal loss following early postnatal alcohol exposure.
Differential neuronal loss following early postnatal alcohol exposure.
复制标题
产后早期酒精暴露后神经元的差异性损失。
DOI：
10.1002/tera.1420400205
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
West,JR
中科院分区：
文献类型：
--
作者：
Pierce,DR;Goodlett,CR;West,JR
新生大鼠在出生后第4 - 10天每天暴露于6.6 g/kg酒精，同时使用人工饲养程序，每天产生高峰和低谷血液酒精浓度。胃造口对照组人工饲养，用麦芽糖/糊精等热量替代牛奶配方中的酒精，而胃造口对照组由母鼠正常饲养。在第10天处死幼仔，并在小脑矢状面和海马结构水平面上获得组织切片（2 μm厚）。整体面积的措施，获得适当的海马，齿状区，小脑，沿着这些地区的细胞层的面积。在海马结构中，细胞计数由海马固有的锥体细胞、门部的多种细胞类型和齿状区的颗粒细胞组成。在小脑中，从小叶I、V、VII、VIII和IX获得浦肯野细胞、颗粒层颗粒细胞、外颗粒层颗粒细胞和外颗粒层有丝分裂细胞的细胞计数。酒精选择性地减少小脑的面积和神经元数量，但对海马结构的神经元数量没有显着影响。浦肯野细胞表现出最大的百分比减少，小脑颗粒细胞显着减少的颗粒层，但不是在外部颗粒层。所有的小叶都显示出这些效应，但小叶I比分析的其他四个小叶受到的影响更大。结果表明，所选择的神经元群体的差异脆弱性，在大脑生长突增期酒精暴露的发育毒性。
Neonatal rats were exposed to 6.6 g/kg of alcohol each day between postnatal days 4 and 10 while artificial‐rearing procedures were used, in a manner which produced high peak and low trough blood alcohol concentrations each day. Gastrostomy controls were reared artificially with maltose/dextrin isocalorically substituted for alcohol in the milk formula, and suckle controls were reared normally by dams. The pups were sacrificed on day 10 and tissue sections (2 μm thick) were obtained in the sagittal plane through the cerebellum and in the horizontal plane through the hippocampal formation. Overall area measures were obtained for the hippocampus proper, area dentata, and cerebellum, along with areas of the cell layers of these regions. In the hippocampal formation, cell counts were made of the pyramidal cells of the hippocampus proper, the multiple cell types of the hilus, and the granule cells of the area dentata. In the cerebellum, cell counts of Purkinje cells, granule cells of the granular layer, granule cells of the external granular layer, and mitotic cells of the external granular layer were obtained from lobules I, V, VII, VIII, and IX. Alcohol selectively reduced areas and neuronal numbers in the cerebellum but had no significant effects on neuronal numbers in the hippocampal formation. Purkinje cells exhibited the greatest percent reductions, and cerebellar granule cells were significantly reduced in the granular layer but not in the external granular layer. All lobules showed these effects, but lobule I was significantly more affected than the other four lobules that were analyzed. The results demonstrate the differential vulnerability of selected neuronal populations to the developmental toxicity of alcohol exposure during the brain growth spurt.