A novel animal model of underactive bladder : Analysis of lower urinary tract function in a rat lumbar canal stenosis model
A novel animal model of underactive bladder : Analysis of lower urinary tract function in a rat lumbar canal stenosis model
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新型膀胱活动不足动物模型：大鼠腰管狭窄模型下尿路功能分析
DOI：
10.1002/nau.21255
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Nishiyama H
中科院分区：
文献类型：
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作者：
Sekido N;Joraku A;Okada H;Wakamatsu D;Matsuya H;Nishiyama H
目的神经源性膀胱活动不全（UAB）的动物模型尚未建立。据报道，通过马尾压迫建立的大鼠腰椎管狭窄（LCS）模型出现间歇性跛行和异常性疼痛。在本研究中，我们检查了大鼠LCS模型的下尿路功能。方法在第五腰椎弓（假手术）处钻一个小孔，并将一块矩形硅橡胶插入L5-L6硬膜外间隙（LCS）。手术前后进行了代谢笼研究。手术后，进行了清醒膀胱测压（CMG）和体外肌肉条带研究。采用苏木精和伊红染色评价膀胱形态。结果与Sham大鼠相比，LCS大鼠排尿量显着减少，排尿后残余量和残余尿率显着增加。 CMG显示排尿后残余尿量和非排尿收缩次数显着增加，而排尿时的排尿量、阈压和最大膀胱内压显着下降。假手术大鼠和 LCS 大鼠对卡巴胆碱的反应没有显着差异。相比之下，LCS 大鼠对场刺激的反应显着增加，尤其是在较低频率下。 LCS大鼠逼尿肌形态无明显差异。结论该大鼠模型手术操作相对简单，具有神经源性UAB的特点。它似乎有助于阐明 UAB 的病理生理学，并且可能具有评估 UAB 药物治疗的潜力。神经尿素。尿动力学。 31:1190–1196，2012 年。© 2012 Wiley periodicals, Inc.
AimsAn animal model of neurogenic underactive bladder (UAB) has not been established. It was reported that a rat lumbar spinal canal stenosis (LCS) model created by cauda equina compression manifested intermittent claudication and allodynia. In this study, we examined the lower urinary tract function of the rat LCS model.MethodsOne small hole was drilled at the fifth lumbar vertebral arch (sham), and a rectangular piece of silicone rubber was inserted into the L5–L6 epidural space (LCS). Before and after surgery, a metabolic cage study was performed. After surgery, awake cystometry (CMG) and an in vitro muscle strip study were performed. Bladder morphology was evaluated by hematoxylin and eosin staining.ResultsThe LCS rats showed a significant decrease in voided volume and a significant increase in postvoid residual volume and residual urine rate compared with Sham rats. CMG showed that the postvoid residual urine volume and numbers of non‐voiding contractions significantly increased, while the voided volume, threshold pressure, and maximum intravesical pressure during voiding significantly decreased. There were no significant differences between sham and LCS rats in response to carbachol. In contrast, there was a significant increase in response to field stimulation, especially at lower frequencies, in LCS rats. LCS rats showed no obvious difference in detrusor morphology.ConclusionsThis rat model requires a relatively simple surgical procedure and has characteristics of neurogenic UAB. It seems to be useful in the pathophysiological elucidation of UAB and might have potential for assessment of pharmacotherapy of UAB. Neurourol. Urodynam. 31:1190–1196, 2012. © 2012 Wiley Periodicals, Inc.