Effect of cryoprotectants, equilibration periods and freezing rates on cryopreservation of spermatozoa of mahseer, Tor khudree (Sykes) and T-putitora (Hamilton)
Effect of cryoprotectants, equilibration periods and freezing rates on cryopreservation of spermatozoa of mahseer, Tor khudree (Sykes) and T-putitora (Hamilton)
复制标题
DOI：
10.1111/j.1365-2109.2005.01331.x
复制
发表时间：
2005-11-15
影响因子：
2
通讯作者：
Lakra, WS
中科院分区：
文献类型：
--
作者：
Patil, R;Lakra, WS
本研究旨在规范濒危物种马塞鱼Tor khudree（Sykes）和马塞鱼Tor khudree（Sykes）精子的冷冻保存方案。putitora（汉密尔顿）.对冷冻保护剂二甲基亚砜（DMSO）和甘油以及两者组合的适用性进行了测试。还测试了四个平衡期和四个冷冻速率以进行标准化。9%DMSO和11%甘油的组合显著提高了两种T的平均孵化率。khudree（45.59 ± 1.86%）（对照71.08 ± 0.59%）和T. putitora（45.00 ± 1.25%）（对照73.48 ± 1.19%）。在四个不同的平衡时间中，30 min（-1）的平衡时间产生的平均孵化率最高。khudree（39.46 ± 1.94%）（对照71.70 ± 0.61%）和T.恶臭（38.28 ± 1.06%）（对照组73.11 ± 0.82%）。在液氮表面上方8 cm处冷冻10 min（-1），两种蝌蚪的孵化率较高。khudree（41.75 ± 1.72%）（对照73.99 ± 1.17%）和T. putitora（41.34 ± 2.04%）（对照组72.48 ± 1.51%）。该研究首次报告了DMSO和甘油组合的上级性能。
A study was conducted to standardize a protocol for cryopreservation of spermatozoa of the endangered mahseer, Tor khudree (Sykes) and T. putitora (Hamilton). The suitability of the cryoprotectants, dimethyl sulphoxide (DMSO) and glycerol, and the combination of the two were tested. Four equilibration periods and four freezing rates were also tested for their standardization. A combination of 9% DMSO and 11% glycerol gave significantly higher mean percentage of hatching in both T. khudree (45.59 +/- 1.86%) (control 71.08 +/- 0.59%) and T. putitora (45.00 +/- 1.25%) (control 73.48 +/- 1.19%) among the eight different treatments. Among the four different equilibration periods tested, the equilibration period of 30 min(-1) yielded the highest mean hatching percentage in T. khudree (39.46 +/- 1.94%) (control 71.70 +/- 0.61%) and T. putitora (38.28 +/- 1.06%) (control 73.11 +/- 0.82%). Freezing straws at a height of 8 cm above LN2 surface for 10 min(-1) gave higher hatching percentages for both T. khudree (41.75 +/- 1.72%) (control 73.99 +/- 1.17%) and T. putitora (41.34 +/- 2.04%) (control 72.48 +/- 1.51%). The study reports the superior performance of the combination of DMSO and glycerol for the first time.