Veillonella Catalase Protects the Growth of Fusobacterium nucleatum in Microaerophilic and Streptococcus gordonii-Resident Environments.
Veillonella Catalase Protects the Growth of Fusobacterium nucleatum in Microaerophilic and Streptococcus gordonii-Resident Environments.
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韦荣球菌过氧化氢酶可保护微需氧和戈登链球菌居住环境中具核梭杆菌的生长。
DOI：
10.1128/aem.01079-17
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发表时间：
2017
影响因子：
4.4
通讯作者：
Qi,Fengxia
中科院分区：
文献类型：
--
作者：
Zhou,Peng;Li,Xiaoli;Huang,I-Hsiu;Qi,Fengxia
口腔生物膜是一个多物种的群落，在这个群落中，敌友之间存在着对抗和互惠，以维持群落成员的生态平衡。先锋定殖菌，如戈登链球菌，产生H2 O2来抑制竞争者的生长，如变形链球菌，以及牙齿生物膜的严格厌氧中期和晚期定殖菌。有趣的是，韦荣氏球菌属物种，作为早期殖民者，物理相互作用（共聚集）与S。gordonii。一个假定的过氧化氢酶基因（catA）被发现在大多数测序韦荣氏球菌物种，然而，这个基因的功能是未知的。在这项研究中，我们通过将小韦荣球菌PK 1910的catA整合到唯一的可转化菌株，Veillonella vacuica OK 5中来表征catA的生态功能，该菌株是catA阴性的。菌株（OK 5-catA）对H2 O2的抗性增强。进一步的研究表明，thecatA基因的表达可通过加入H2 O2或与S. gordonii。混合培养实验进一步表明，转基因OK 5-catA菌株不仅在微需氧条件下促进具核梭杆菌（一种严格厌氧的牙周病病原体）的生长，而且还拯救了F. S. nucleatum的杀伤作用。gordonii。过氧化氢酶在韦荣球菌生物膜生态和发病机制中的潜在作用进行了讨论hower.IMPORTANCEVeillonella物种，作为早期殖民者，可以与许多细菌，包括最初的殖民者链球菌戈登和牙周病原体具核梭杆菌，在口腔生物膜形成的各个阶段共聚集。除了为许多微生物提供结合位点外，我们以前的研究还表明韦荣氏球菌为牙周病原体的生存和生长提供营养。这些发现表明韦荣氏球菌在口腔生物膜的形成和人类口腔生态学中起着重要的“桥梁”作用。在这项研究中，我们证明了韦荣氏球菌的还原活性不仅可以在微需氧条件下拯救具核梭杆菌的生长，而且可以在戈登链球菌存在的环境中拯救具核梭杆菌的生长。本研究为进一步研究口腔生物膜的形成机制和牙周疾病的防治提供了新的思路。
The oral biofilm is a multispecies community in which antagonism and mutualism coexist among friends and foes to keep an ecological balance of community members. The pioneer colonizers, such as Streptococcus gordonii, produce H2O2to inhibit the growth of competitors, like the mutans streptococci, as well as strict anaerobic middle and later colonizers of the dental biofilm. Interestingly, Veillonella species, as early colonizers, physically interact (coaggregate) with S. gordonii. A putative catalase gene (catA) is found in most sequenced Veillonella species; however, the function of this gene is unknown. In this study, we characterized the ecological function ofcatAfrom Veillonella parvula PK1910 by integrating it into the only transformable strain, Veillonella atypica OK5, which iscatAnegative. The strain (OK5-catA) became more resistant to H2O2. Further studies demonstrated that thecatAgene expression is induced by the addition of H2O2or coculture with S. gordonii. Mixed-culture experiments further revealed that the transgenic OK5-catAstrain not only enhanced the growth of Fusobacterium nucleatum, a strict anaerobic periodontopathogen, under microaerophilic conditions, but it also rescued F. nucleatum from killing by S. gordonii. A potential role of catalase in veillonellae in biofilm ecology and pathogenesis is discussed here.IMPORTANCEVeillonella species, as early colonizers, can coaggregate with many bacteria, including the initial colonizer Streptococcus gordonii and periodontal pathogen Fusobacterium nucleatum, during various stages of oral biofilm formation. In addition to providing binding sites for many microbes, our previous study also showed that Veillonella produces nutrients for the survival and growth of periodontal pathogens. These findings indicate that Veillonella plays an important “bridging” role in the development of oral biofilms and the ecology of the human oral cavity. In this study, we demonstrated that the reducing activity of Veillonella can rescue the growth of Fusobacterium nucleatum not only under microaerophilic conditions, but also in an environment in which Streptococcus gordonii is present. Thus, this study will provide a new insight for future studies on the mechanisms of human oral biofilm formation and the control of periodontal diseases.