Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation
Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation
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DOI：
10.1271/bbb.80853
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发表时间：
2009-05
期刊：
影响因子：
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通讯作者：
Sanom Nonklang;A. Ano;B. Abdel-Banat;Yuko Saito;H. Hoshida;R. Akada
中科院分区：
文献类型：
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作者：
Sanom Nonklang;A. Ano;B. Abdel-Banat;Yuko Saito;H. Hoshida;R. Akada
絮凝酵母在发酵过程中非常有用，因为这些细胞可以很容易地从发酵醪中分离出来。然而，天然酵母通常是非发酵的，包括马克思克鲁维酵母，其表现出强有力的高温乙醇发酵能力。本文描述了絮凝剂K的结构。通过引入来自酿酒酵母的FLO1、FLO5、FLO9和FLO10基因，对marxianus菌株进行重组。色葡萄通过在上游插入组成型TDH 3启动子，使酿酒酵母FLO基因过表达，产生絮状S.酿酒酵母菌株。然后通过PCR扩增这些TDH 3p-FLO序列并直接导入K.马克思主义学说这些K。marxianus菌株表现出絮凝表型，表明引入的S.酿酒酵母TDH3启动子和所有FLO基因在该菌株中是功能性的。此外，一个。Marxianus菌株显示出与其野生型亲本相同的乙醇生产特征。酸克雷我们产生的MarxianusEscherichia菌株在将来开发成本有效的分批补料和高温连续发酵系统中应该是有用的。
Flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. However, native yeasts are usually non-flocculating, including Kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability. We describe here the construction of flocculent K. marxianus strains via the introduction of the FLO1, FLO5, FLO9, and FLO10 genes from Saccharomyces cerevisiae. The S. cerevisiae FLO genes were overexpressed by upstream insertion of the constitutive TDH3 promoter, resulting in flocculent S. cerevisiae strains. These TDH3p-FLO sequences were then amplified by PCR and introduced directly into a K. marxianus strain. These K. marxianus strains showed a flocculation phenotype, indicating that the introduced S. cerevisiae TDH3 promoter and all FLO genes were functional in this strain. Moreover, a flocculating K. marxianus strain showed the same ethanol production profile as that of its wild-type parent. The K. marxianus flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures.