Signalling through neutrophil Fc gamma RIII, Fc gamma RII, and CD59 is not impaired in active rheumatoid arthritis.
Signalling through neutrophil Fc gamma RIII, Fc gamma RII, and CD59 is not impaired in active rheumatoid arthritis.
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在活动性类风湿性关节炎中，通过中性粒细胞 Fc gamma RIII、Fc gamma RII 和 CD59 发出的信号并未受损。
DOI：
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发表时间：
1996
影响因子：
27.4
通讯作者：
Paul Morgan
中科院分区：
文献类型：
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作者：
Jane Jones;I. Laffafian;Tom Lawson;Bryan D. Williams;Paul Morgan
目的：比较中性粒细胞Fc受体（Fc γ R）和CD59在正常健康受试者和活动性类风湿关节炎（RA）患者中的信号反应。方法：用荧光荧光计测定负载荧光钙指示剂fura-2的中性粒细胞细胞内游离钙浓度。结果：当不添加一抗、CD59或CD32 （Fc gamma RIII）抗体时，两组细胞内钙离子的基础浓度相似。当添加CD16 （Fc gamma RIII）抗体时，患者组的基础钙浓度明显高于对照组。将Fc γ RII、Fc γ RIII或CD59与特异性单克隆抗体结合并与羊抗小鼠IgG的F(ab)2片段交联后，观察到瞬时胞质钙离子通量。三种受体交联后，细胞内游离钙[Ca2+]i的峰值浓度在正常健康供体和RA患者之间具有可比性。添加交联抗体与钙增加之间的滞后期在正常个体和患者之间也是相似的。结论：与先前的报道相反，这些结果表明，RA患者血液中性粒细胞中交联Fc受体的Ca2+信号反应与正常受试者的中性粒细胞相同。交联CD59的信号反应也没有改变。
OBJECTIVE: To compare neutrophil Fc receptor (Fc gamma R) and CD59 signalling responses in normal healthy subjects and patients with active rheumatoid arthritis (RA). METHODS: Intracellular free calcium concentrations were measured in neutrophils loaded with the fluorescent calcium indicator fura-2, using a spectrofluorimeter. RESULTS: Basal intracellular calcium ion concentrations were similar in both groups when no primary antibody, CD59, or CD32 (Fc gamma RIII) antibody was added. When CD16 (Fc gamma RIII) antibody was added, there was a significantly greater basal calcium concentration in the patient group compared with the control group. Transient cytosolic calcium ion fluxes were observed after binding Fc gamma RII, Fc gamma RIII, or CD59 with specific monoclonal antibodies and cross linking with the F(ab)2 fragment of sheep antimouse IgG. Peak concentrations of intracellular free calcium, [Ca2+]i, after cross linking each of the three receptors, were comparable between normal healthy donors and patients with RA. The lag period between addition of cross linking antibodies and the increase in calcium was also similar between normal individuals and patients. CONCLUSION: Contrary to previous reports, these results demonstrate that Ca2+ signalling responses of cross linked Fc receptors in blood neutrophils from patients with RA are identical to those in neutrophils of normal subjects. Signalling responses of cross linked CD59 are also unaltered.