Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.
Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.
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在胎儿和新生儿期间暴露于柴油机排气的大鼠中，血清免疫球蛋白E升高到japonica japonica花粉中。
DOI：
10.1186/1471-2393-2-2
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发表时间：
2002-03-25
影响因子：
3.1
通讯作者：
Ohsawa, Masanobu
中科院分区：
文献类型：
--
作者：
Watanabe, Nobue;Ohsawa, Masanobu
研究了胎儿期柴油机废气吸入对出生后抗花粉IgE升高的影响。该实验是用大鼠幼仔进行的。组我;暴露在洁净空气中（管制）。2、3组；胎儿期暴露于总或过滤柴油废气（total - c - c, filtered - c - c）。4、5组；在哺乳期间暴露于总或过滤的柴油废气（C-Total-C, C-Filtered-C）。组6、7;在断奶期间暴露在总或过滤的柴油废气中（C-C-Total, C-C-Filtered）。柴油机排气总颗粒物含量为1.73 mg/m3，二氧化氮含量为0.79 ppm；过滤后的废气与不含颗粒物的总废气含有相同的气体。从出生后第49天开始，每隔2周腹腔注射粗雪松花粉5 mg。第四次免疫后，对照组、Total-C-C、Filtered-C-C、C-Total-C、C-Filtered-C、C-C-Total和C-C-Filtered的平均IgE滴度分别为64.0±2.7、469.5±1.6、332.0±1.7、380.4±1.7、394.8±1.7、115.9±1.3和57.0±2.8。Total-C-C、Filtered-C-C、C-Total-C、C-Filtered-C与Control之间差异有统计学意义（p < 0.01, p < 0.01, p < 0.05, p < 0.01）。ELISA检测的平均IgE滴度分别为0.47±0.06、0.79±0.35、0.86±0.46、0.80±0.22、0.56±0.08、0.46±0.04、0.45±0.03。滤过c - c和C-Total-C组的IgE滴度均显著高于对照组（p < 0.05）。在免疫系统分化过程中吸入柴油废气加速了针对花粉的IgE的升高。
The possible participation of diesel exhaust inhalation during the fetal period in the elevation of IgE against pollen in postnatal life was investigated. The experiment was conducted using rat pups. Group I; exposed to clean air (Control). Group 2, 3; exposed to total or filtered diesel exhaust during the fetal period (Total-C-C, Filtered-C-C). Group 4, 5; exposed to total or filtered diesel exhaust during the suckling period (C-Total-C, C-Filtered-C). Group 6,7; exposed to total or filtered diesel exhaust during the weaning period (C-C-Total, C-C-Filtered). Total diesel engine exhaust contained 1.73 mg/m3 particulate matter and 0.79 ppm nitrogen dioxide; filtered exhaust contained the same gases as the total exhaust without particulate matter. Intraperitoneal injection of 5 mg crude cedar pollen was performed at 2-week intervals from the 49th day after birth. The mean IgE titers measured by the P-K reaction in the Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 respectively after the fourth immunization. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). The mean IgE titers by ELISA were 0.47 ± 0.06, 0.79 ± 0.35, 0.86 ± 0.46, 0.80 ± 0.22, 0.56 ± 0.08, 0.46 ± 0.04 and 0.45 ± 0.03, respectively. IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each). Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.