Peripheral blood neutrophil cytokine hyper-reactivity in chronic periodontitis
Peripheral blood neutrophil cytokine hyper-reactivity in chronic periodontitis
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DOI：
10.1177/1753425915589387
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发表时间：
2015-10-01
期刊：
影响因子：
3.2
通讯作者：
Matthews, John B.
中科院分区：
文献类型：
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作者：
Ling, Martin R.;Chapple, Iain L. C.;Matthews, John B.
在存在/不存在大肠杆菌LPS、调理的金黄色葡萄球菌、热灭活的具核梭杆菌和牙龈卟啉单胞菌的情况下培养18小时后，测定从牙周炎患者（治疗前/后）和匹配对照中分离的外周血中性粒细胞的促炎细胞因子释放（IL-8、IL-6、TNF-α、IL-1）。所有培养物显示出检测到的每种细胞因子的量的差异（PIL-6>TNF-=IL-1）。未受刺激的患者中性粒细胞释放的细胞因子中位数始终高于对照细胞，但无显著性差异。未经治疗的患者中性粒细胞的刺激细胞因子释放也始终高于对照细胞。当所有刺激的数据结合起来时，这种高反应性对所有测试的细胞因子都是显著的（P
Pro-inflammatory cytokine release (IL-8, IL-6, TNF-,IL-1) by peripheral blood neutrophils, isolated from periodontitis patients (before/after therapy) and matched controls, was determined after 18h culture in the presence/absence of Escherichia coli LPS, opsonised Staphylococcus aureus, heat-killed Fusobacterium nucleatum and Porphyromonas gingivalis. All cultures demonstrated differences in the amounts of each cytokine detected (PIL-6>TNF-=IL-1). Median cytokine release from unstimulated patient neutrophils was consistently, but non-significantly, higher than from control cells. Stimulated cytokine release from untreated patient neutrophils was also consistently higher than from control cells. This hyper-reactivity was significant for all tested cytokines when data for all stimuli were combined (P