Authentication of medicinal herbs using PCR-Amplified ITS2 with specific primers
Authentication of medicinal herbs using PCR-Amplified ITS2 with specific primers
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DOI：
10.1055/s-2007-990227
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发表时间：
2007-10-01
期刊：
影响因子：
2.7
通讯作者：
Lin, Tsai-Yun
中科院分区：
文献类型：
--
作者：
Chiou, Shu-Jiau;Yen, Jul-Hung;Lin, Tsai-Yun
长期以来，中草药的不同部位被用作治疗多种疾病的中药，而具有相似形态和化学指纹图谱的材料经常被错误识别。核糖体DNA（rDNA）内转录间隔区（ITS）序列变异分析已成为从基因间和种间水平鉴定中草药的有效方法。从加工材料中提取的DNA通常被微生物严重降解或污染，因此不会产生或产生意外的PCR产物。本研究的目的是利用设计的两对引物选择性扩增ITS片段，用于高效、准确地鉴定中药材。用所设计的引物对ITS 2基因的特异区域进行PCR扩增，并对48科55种炮制药材的DNA进行验证。此外，选择性扩增的ITS 2鉴定了5组易混淆的中草药材料。所设计的引物适用于中药材的鉴别。
Different parts of medicinal herbs have long been used as traditional Chinese drugs for treating many diseases, whereas materials of similar morphology and chemical fingerprints are often misidentified. Analyses of sequence variations in the nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) have become a valid method for authentication of medicinal herbs at the intergenic and interspecific levels. DNA extracted from processed materials is usually severely degraded or contaminated by microorganisms, thus generates no or unexpected PCR products. The goal of this study is to apply the ITS fragments selectively amplified with two designed primer sets for efficient and precise authentication of medicinal herbs. The designed primers led to an accurate PCR product of the specific region in ITS2, which was confirmed with DNA extracted from 55 processed medicinal herbs belonging to 48 families. Moreover, the selectively amplified ITS2 authenticated five sets of easily confusable Chinese herbal materials. The designed primers were proven to be suitable for a broad application in the authentication of herbal materials.