Sphingobacterium sp strain PM2-P1-29 harbours a functional tet(X) gene encoding for the degradation of tetracycline
Sphingobacterium sp strain PM2-P1-29 harbours a functional tet(X) gene encoding for the degradation of tetracycline
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DOI：
10.1111/j.1365-2672.2008.04101.x
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发表时间：
2009-04-01
影响因子：
4
通讯作者：
LaPara, T. M.
中科院分区：
文献类型：
--
作者：
Ghosh, S.;Sadowsky, M. J.;LaPara, T. M.
泰特（X）基因先前已在专性厌氧拟杆菌属中发现，这很奇怪，因为泰特（X）编码NADP依赖性单加氧酶，该酶需要氧气来降解四环素。在这项研究中，我们确定了一个四环素耐药，好氧，革兰氏阴性鞘氨醇杆菌属菌株PM 2-P1-29，携带一个泰特（X）基因。鞘氨醇杆菌属PM 2-P1-29显示出与杀死的对照相比转化四环素的能力。通过PCR和核苷酸序列分析证实了泰特（X）基因的存在。对泰特（X）基因侧翼区域的额外核苷酸序列分析揭示了可移动的转座子样元件（Tn 6031），其与先前描述的拟杆菌属接合转座子CTnDOT共享组织特征和基因。检测到泰特（X）区域的环状转座中间体，这是可移动转座子的特征。然而，我们不能证明泰特（X）基因的接合转移使用三种不同的受体菌株和众多的实验条件。这项研究表明，鞘氨醇杆菌属PM 2-P1-29或相关细菌可能是泰特（X）基因的祖先来源。这项研究表明，环境细菌和横向基因转移在细菌之间的抗生素耐药性的传播和增殖的重要性。
The tet(X) gene has previously been found in obligate anaerobic Bacteroides spp., which is curious because tet(X) encodes for a NADP-dependent monooxygenase that requires oxygen to degrade tetracycline. In this study, we characterized a tetracycline resistant, aerobic, Gram-negative Sphingobacterium sp. strain PM2-P1-29 that harbours a tet(X) gene.Sphingobacterium sp. PM2-P1-29 demonstrated the ability to transform tetracycline compared with killed controls. The presence of the tet(X) gene was verified by PCR and nucleotide sequence analysis. Additional nucleotide sequence analysis of regions flanking the tet(X) gene revealed a mobilizable transposon-like element (Tn6031) that shared organizational features and genes with the previously described Bacteroides conjugative transposon CTnDOT. A circular transposition intermediate of the tet(X) region, characteristic of mobilizable transposons, was detected. However, we could not demonstrate the conjugal transfer of the tet(X) gene using three different recipient strains and numerous experimental conditions.This study suggests that Sphingobacterium sp. PM2-P1-29 or a related bacterium may be an ancestral source of the tet(X) gene.This study demonstrates the importance of environmental bacteria and lateral gene transfer in the dissemination and proliferation of antibiotic resistance among bacteria.