Mps1 promotes poleward chromosome movements in meiotic prometaphase.
Mps1 promotes poleward chromosome movements in meiotic prometaphase.
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DOI：
10.1091/mbc.e20-08-0525-t
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发表时间：
2021-05-01
影响因子：
3.3
通讯作者：
Dawson DS
中科院分区：
文献类型：
--
作者：
Meyer RE;Tipton AR;LaVictoire R;Gorbsky GJ;Dawson DS
在减数分裂I前期，同源染色体配对并通过交换连接。交叉，交叉形成的连接，使染色体对，称为二价体，作为一个单一的单位附着在纺锤体上。当减数分裂纺锤体在前中期形成时，大多数二价体与一个纺锤体极结合，然后在纺锤体上经历一系列振荡，附着在微管上并从微管上分离，直到二价体的配偶体变成生物定向的-从纺锤体的相对两侧附着在微管上。保守的激酶Mps 1对于二价体在前中期被微管拉过纺锤体是必不可少的。在这里，我们表明，MPS 1是需要有效触发微管附着的动粒向两极迁移，并促进微管解聚。我们的数据支持模型Mps 1在动粒处起作用，以协调微管的成功附着和微管解聚的触发，然后移动染色体。
In prophase of meiosis I, homologous chromosomes pair and become connected by cross-overs. Chiasmata, the connections formed by cross-overs, enable the chromosome pair, called a bivalent, to attach as a single unit to the spindle. When the meiotic spindle forms in prometaphase, most bivalents are associated with one spindle pole and then go through a series of oscillations on the spindle, attaching to and detaching from microtubules until the partners of the bivalent become bioriented—attached to microtubules from opposite sides of the spindle. The conserved kinase, Mps1, is essential for the bivalents to be pulled by microtubules across the spindle in prometaphase. Here we show that MPS1 is needed for efficient triggering of the migration of microtubule-attached kinetochores toward the poles and promotes microtubule depolymerization. Our data support the model Mps1 acts at the kinetochore to coordinate the successful attachment of a microtubule and the triggering of microtubule depolymerization to then move the chromosome.