Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka
Effects of constitutive expression of somatolactin alpha on skin pigmentation in medaka
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DOI：
10.1016/j.gene.2009.04.010
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发表时间：
2009-08-01
期刊：
影响因子：
3.5
通讯作者：
Kinoshita, Masato
中科院分区：
文献类型：
--
作者：
Fukamachi, Shoji;Yada, Takashi;Kinoshita, Masato
生长激素α（SLa）是一种鱼类特有的肽类激素，由脑垂体分泌。它的功能在过去十年中一直在研究，但仍在辩论中。我们以前报道了一个青鳉突变体，颜色干扰（ci），这表明在身体颜色调节，脂质储存和皮质醇分泌的缺陷SLa的移码突变。在这项研究中，我们研究了引入的DNA片段，组成型表达野生型SLa的β-肌动蛋白（Actb）启动子的调控下到ci基因组的影响。我们利用内部核糖体进入位点（IRES）和绿色荧光蛋白（GFP）成功地可视化了转基因表达。转基因青鳉，Actb-SLa：GFP，表现出相反的体色表型ci;更多的橙子黄色素和更少的白色白色素。我们还检测到更多的黑色黑色素细胞和更少的银虹膜细胞，这表明SLa在调节皮肤中所有类型的色素细胞中的综合作用。出乎意料的是，脂质/皮质醇含量的缺陷保留在Actb-SLa：GFP中。因此，SLa和脂质/皮质醇代谢之间的因果关系复发到一个开放的问题，需要重新评估其他类型的实验。Actb-SLa：GFP和ci鱼的生长和成熟与野生型相似，表明SLa对生长调节的贡献很小，尽管它在体外与硬骨鱼特异性生长激素受体结合。本研究提供了明确的证据，SLa的主要和不可或缺的作用，在青少年的体色调节。(C)2009 Elsevier B. V.保留所有权利。
Somatolactin alpha (SLa) is a fish-specific peptide hormone secreted from the pituitary. Its functions have been investigated for the last decade but are still under debate. We previously reported a frame-shift mutation on SLa in a medaka mutant, color interfere (ci), which shows defects in body-color regulation, lipid storage, and cortisol secretion. In this study, we examined the effects of introducing a DNA fragment which constitutively expresses wild-type SLa under regulation of the beta-actin (Actb) promoter into the ci genome. We successfully visualized the transgene expression by taking advantage of an internal ribosome entry site (IRES) and the green fluorescent protein (GFP). The transgenic medaka, Actb-SLa:GFP, exhibited a reversed body-color phenotype of ci; more orange xanthophores and less white leucophores. We also detected more black melanophores and less silver iridophores, which indicates SLa's comprehensive role in regulating all types of pigment cells in the skin. Unexpectedly, the defects in lipid/cortisol contents remained in Actb-SLa:GFP. Therefore, the causal relationship between SLa and lipid/cortisol metabolisms relapses to an open question which needs to be reassessed by other types of experiments. Both the Actb-SLa:GFP and ci fish grow and mature similar to wild type, indicating SLa contributes little to growth regulation in spite of the fact that it binds to a teleost-specific paralog of growth-hormone receptor in vitro. The present study provides definitive evidence for SLa's principal and indispensable role in body-color regulation in medaka. (C) 2009 Elsevier B.V. All rights reserved.