LncRNA HAND2-AS1 inhibits proliferation and promotes apoptosis of non-small cell lung cancer cells by inactivating PI3K/Akt pathway.
LncRNA HAND2-AS1 inhibits proliferation and promotes apoptosis of non-small cell lung cancer cells by inactivating PI3K/Akt pathway.
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LncRNA HAND2-AS1通过失活PI3K/Akt通路抑制非小细胞肺癌细胞增殖，促进细胞凋亡。
DOI：
10.1042/bsr20201870
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发表时间：
2020-11-27
影响因子：
4
通讯作者：
Zhao P
中科院分区：
文献类型：
--
作者：
Gao T;Dai X;Jiang Y;He X;Yuan S;Zhao P
背景：非小细胞肺癌（Non-small cell lung cancer，NSCLC）是肺癌的主要亚型，发病率和死亡率高。lncRNA HAND 2-AS 1的功能仅在子宫内膜样癌和骨肉瘤中报道。在我们的研究中，研究了HAND 2-AS 1在NSCLC中的作用。方法：采用qRT-PCR方法检测HAND 2-AS 1在NSCLC患者和健康对照者肺组织和血清中的表达。采用卡方检验分析HAND 2-AS 1表达水平与NSCLC患者临床资料的相关性。CCK-8法检测细胞增殖，细胞凋亡检测细胞凋亡，Western blot检测PI 3 K/Akt通路相关蛋白表达。结果：HAND 2-AS 1在NSCLC中的表达明显下调。HAND 2-AS 1与NSCLC患者肿瘤大小密切相关。血清HAND 2-AS 1可有效区分骨肉瘤患者和健康对照者，并可用于预测骨肉瘤患者的预后。HAND 2-AS 1过表达可抑制骨肉瘤细胞增殖，促进细胞凋亡，下调PI 3 K/Akt通路相关蛋白的磷酸化水平。PI 3 K/Akt通路抑制剂对HAND 2-AS 1表达无明显影响，但可降低其对细胞增殖和凋亡的影响。结论：HAND 2-AS 1可能通过靶向PI 3 K/Akt通路抑制NSCLC细胞增殖。
Background: Non-small cell lung cancer (NSCLC) is a major subtype of lung cancer and is correlated with high incidence and mortality rate. Functionality of lncRNA HAND2-AS1 is only reported in endometrioid endometrial carcinoma and osteosarcoma. In our study, the role of HAND2-AS1 in NSCLC was investigated. Methods: We first detected the expression of HAND2-AS1 in lung tissues and serum of both NSCLC patients and healthy controls by qRT-PCR. Correlation between HAND2-AS1 expression level and clinical data of NSCLC patients was analyzed by Chi-square test. NSCLC cells, and cell proliferation, cell apoptosis and expression of PI3K/Akt pathway-related proteins were detected by CCK-8 assay, cell apoptosis assay and Western blot, respectively. Results: HAND2-AS1 expression was significantly down-regulated in NSCLC. HAND2-AS1 and tumor size of NSCLC patients were closely associated. Serum HAND2-AS1 can be used to effectively distinguish osteosarcoma patients from healthy controls, and it can also be used to predict prognosis of osteosarcoma patients. HAND2-AS1 overexpression inhibited osteosarcoma cell proliferation, promoted cell apoptosis, and down-regulated phosphorylation of PI3K/Akt pathway-related proteins. PI3K/Akt pathway inhibitor showed no significant effects on HAND2-AS1 expression, but reduced its effects on cell proliferation and apoptosis. Conclusion: We conclude that HAND2-AS1 may suppress the proliferation of NSCLC cells by targeting PI3K/Akt pathway.