Imaging dopamine receptors in humans with [11C]-(+)-PHNO: Dissection of D3 signal and anatomy
Imaging dopamine receptors in humans with [11C]-(+)-PHNO: Dissection of D3 signal and anatomy
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DOI：
10.1016/j.neuroimage.2010.06.044
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发表时间：
2011-01-01
期刊：
影响因子：
5.7
通讯作者：
Gunn, Roger N.
中科院分区：
文献类型：
--
作者：
Tziortzi, Andri C.;Searle, Graham E.;Gunn, Roger N.
[C-11]-(+)- phno是一种D3偏好PET放射配体，最近开辟了在体内人脑中成像D3受体的可能性。这种成像工具可以表征D3受体在体内的分布，并进一步研究它们的功能作用。特异的[C-11]-(+)- phno信号是D3和D2组分的混合物，每种组分的相对大小由区域受体密度决定。因此，对感兴趣区域（ROI）的准确和可重复的描述对于人类PET数据的最佳分析非常重要。我们提出了一套描述D3相关roi的解剖学指南，包括黑质、下丘脑、腹侧苍白质/无名质、腹侧纹状体、苍白球和丘脑。使用这种方法描述这些结构允许操作者内部和操作者之间的高再现性。随后，我们使用选择性D3拮抗剂将每个区域的[C-11]-(+)-PHNO信号分解为D3和D2组分，并估计D3信号的区域分数(f（PHNO)(D3)）。f (D3)（PHNO）由大到小依次为：下丘脑= 100%，黑质= 100%，腹侧苍白球/无名质= 75%，苍白球= 65%，丘脑= 43%，腹侧纹状体= 26%，交前-腹侧壳核= 6%。对于这些感兴趣的解剖区域的描述的自动化方法也被开发和研究在其再现性和准确性方面。(C) 2010爱思唯尔公司版权所有。
[C-11]-(+)-PHNO is a D3 preferring PET radioligand which has recently opened the possibility of imaging D3 receptors in the human brain in vivo. This imaging tool allows characterisation of the distribution of D3 receptors in vivo and further investigation of their functional role. The specific [C-11]-(+)-PHNO signal is a mixture of D3 and D2 components with the relative magnitude of each component determined by the regional receptor densities. An accurate and reproducible delineation of regions of interest (ROI) is therefore important for optimal analysis of human PET data. We present a set of anatomical guidelines for the delineation of D3 relevant ROIs including substantia nigra, hypothalamus, ventral pallidum/substantia innominata, ventral striatum, globus pallidus and thalamus. Delineation of these structures using this approach allowed for high intra- and inter-operator reproducibility. Subsequently we used a selective D3 antagonist to dissect the total [C-11]-(+)-PHNO signal in each region into its D3 and D2 components and estimated the regional fraction of the D3 signal (f(PHNO)(D3)). In descending order of magnitude the following results for the f (D3)(PHNO) were obtained: hypothalamus = 100%, substantia nigra = 100%, ventral pallidum/substantia innominata = 75%, globus pallidus = 65%, thalamus = 43%, ventral striatum = 26% and precommissural-ventral putamen = 6%. An automated approach for the delineation of these anatomical regions of interest was also developed and investigated in terms of its reproducibility and accuracy. (C) 2010 Elsevier Inc. All rights reserved.