Flow cytometric analysis of head and neck carcinoma DNA index and S-fraction from paraffin-embedded sections: comparison with malignancy grading.
Flow cytometric analysis of head and neck carcinoma DNA index and S-fraction from paraffin-embedded sections: comparison with malignancy grading.
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石蜡包埋切片中头颈癌 DNA 指数和 S 组分的流式细胞术分析：与恶性肿瘤分级的比较。
DOI：
10.1002/cyto.990060511
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Peters,LJ
中科院分区：
文献类型：
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作者：
Johnson,TS;Williamson,KD;Cramer,MM;Peters,LJ
通过流式细胞术分析了73例头颈部表皮样癌患者的存档石蜡包埋病理学标本（代表治疗前组织活检）的DNA指数和%S期细胞，并进行了定量组织形态学评分。来自石蜡包埋标本的DNA荧光/光散射尺寸模式显示与从相同组织标本获得的机械解聚、乙醇固定细胞的DNA荧光/光散射尺寸模式基本相同。模式范围从淋巴细胞样到高度异常DNA指数细胞动力学模式。DNA指数值范围为0.70 - 3.50（中位数1.42），非整倍体频率为63/73（86%）。DNA分布%S范围为4%至45%（平均19），显微镜下恶性肿瘤分级显示出广泛的异质性（平均2.1，范围1.0-3.0，其中1.0-1.7 =高分化，1.8-2.3 =中度分化，2.4-3.0 =低分化）。这些数据的交叉比较显示：（1）肿瘤%S取决于DNA指数（倍性越高，%S越高），（2）低至高恶性肿瘤随机分布在二倍体/近二倍体肿瘤和高度DNA异常肿瘤之间，（3）低至高恶性肿瘤评分肿瘤之间的增殖活性值广泛重叠。然而，那些以高DNA指数（≥ 1.50）和高S期百分比（≥ 20）为特征的癌的高度恶性、浸润性肿瘤的发生率比二倍体/近二倍体（%S期19）肿瘤高5倍。
Archival, paraffin‐embedded, pathology specimens representing pretreatment tissue biopsies from 73 patients with epidermoid carcinoma of the head and neck were analyzed for DNA Index and %S‐phase cells by flow cytometry and were scored for quantitative histomorphology. The DNA fluorescence/light scatter size patterns derived from paraffinembedded specimens were shown to be essentially the same as those from mechanically disaggregated, ethanol‐fixed cells obtained from the same tissue specimen. Patterns ranged from lymphocyte‐like to highly abnormal DNA Index cytokinetic patterns. The DNA Index values ranged from 0.70 to 3.50 (median 1.42), with an aneuploidy frequency of 63/73 (86%). DNA distribution %S ranged from 4% to 45% (mean 19), with the microscopic malignancy grading showing broad heterogeneity (mean 2.1, range 1.0–3.0, where 1.0–1.7 = well differentiated, 1.8–2.3 = moderately differentiated, 2.4–3.0 = poorly differentiated). Cross‐comparison of these data showed that (1) the tumor %S was dependent on DNA Index (higher %S at higher ploidy), (2) low to high malignancy tumors were randomly distributed between diploid/near diploid tumors and high‐degree DNA abnormality tumors, and (3) proliferative activity values broadly overlapped between low to high malignancy scored tumors. However, those carcinomas characterized by high DNA Index (≥ 1.50) and high %S‐phase fractions (≥ 20) had a five fold higher incidence of high‐degree malignancy, invasive tumors than diploid/near diploid (%S ⩽19) tumors.