Glutathione-S-transferase P1 gene polymorphism and susceptibility to endometriosis
Glutathione-S-transferase P1 gene polymorphism and susceptibility to endometriosis
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DOI：
10.1093/humrep/dei053
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发表时间：
2005-08-01
影响因子：
6.1
通讯作者：
Dilek, S
中科院分区：
文献类型：
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作者：
Ertunc, D;Aban, M;Dilek, S
背景：谷胱甘肽-S-转移酶（GST）是Ⅱ相解毒酶系统的关键酶。许多研究探讨了GSTM1和GSTT1基因多态性在子宫内膜异位症中的作用。虽然GSTP1被发现是生殖系统中最丰富的GST类型之一，但关于GSTP1基因多态性在子宫内膜异位症中的作用的重要性的数据不足。方法：本病例对照研究包括150例子宫内膜异位症患者和150名对照。采用PCR和熔解曲线分析方法检测GSTP1单核苷酸多态性的频率。研究结果：内异症组GSTP1ile/ile的比例高于对照组，但差异无统计学意义[比值比（OR）= 1.53; 95%可信区间（CI）= 0.95-2.46]。相反，对照组患者GST 1瓦尔/瓦尔显著升高，似乎对子宫内膜异位症具有保护作用（OR = 0.10; 95%CI = 0.02 - 0.42）。结论：这项研究的结果表明，GST 1基因多态性可能调节子宫内膜异位症的风险，GST 1瓦尔/瓦尔的风险显著降低，GST 1 ile/ile的风险略有增加。进一步研究GST在女性生殖道中的分布及其与疾病的关系，有助于更好地了解GST类型及其多态性在子宫内膜异位症中的作用。
BACKGROUND: Glutathione-S-tranferase (GST) is the part of the key phase II detoxifying enzyme system. Many studies have investigated the role of GSTM1 and GSTT1 gene polymorphisms in endometriosis. Although GSTP1 was found to be one of the most abundant types of GST in genital system, there are insufficient data about the importance of the role of GSTP1 gene polymorphism in endometriosis. METHODS: This case-control study involved 150 patients with endometriosis and 150 controls. The frequency of GSTP1 single nucleotide polymorphisms was evaluated using PCR and melting curve analysis. RESULTS: The proportion of GSTP1 ile/ile tended to be higher in patients with endometriosis than control group, although the difference was not significant [odds ratio (OR)=1.53; 95% confidence interval (CI)=0.95-2.46]. In contrast, GSTP1 val/val was significantly higher in control patients and seems protective for endometriosis (OR=0.10; 95% CI=0.02-0.42). CONCLUSION: The results of this study suggest that GSTP1 polymorphism might modulate the risk of endometriosis with significantly decreased risk for GSTP1 val/val and marginally increased risk for GSTP1 ile/ile. Further studies on not only the disease processes but also normal distribution of the enzyme in female genital tract may provide better understanding about the role of GST types and their polymorphs in endometriosis.