Identification of an Arabidopsis thaliana gene for cardiolipin synthase located in mitochondria
Identification of an Arabidopsis thaliana gene for cardiolipin synthase located in mitochondria
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DOI：
10.1016/j.febslet.2004.10.009
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发表时间：
2004-11-05
期刊：
影响因子：
3.5
通讯作者：
Wada, H
中科院分区：
文献类型：
--
作者：
Katayama, K;Sakurai, I;Wada, H
心磷脂（CL）是一种具有二聚体结构的阴离子磷脂。在真核生物中，它主要位于线粒体的内膜中。虽然CL的生物合成途径是众所周知的，但CL合酶的基因尚未在任何高等生物中鉴定。在这项研究中，CL合酶的CLS基因已被确定在高等植物，拟南芥。我们已经表明，CLS基因编码CL合酶，通过证明其催化从CDP-二酰基甘油和磷脂酰甘油的CL合成反应的能力，并且CLS被靶向到线粒体中。这些结果表明，CLS是一个CL合酶位于线粒体。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Cardiolipin (CL) is an anionic phospholipid with a dimeric structure. In eukaryotes, it is primarily localized in the inner membranes of mitochondria. Although the biosynthetic pathway of CL is well known, the gene for CL synthase has not been identified in any higher organisms. In this study, the CLS gene for a CL synthase has been identified in a higher plant, Arabidopsis thaliana. We have shown that the CLS gene encodes a CL synthase by demonstrating its ability to catalyze the reaction of CL synthesis from CDP-diacylglycerol and phosphatidylglycerol, and that CLS is targeted into mitochondria. These findings demonstrate that CLS is a CL synthase located in mitochondria. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.