Long-chain fatty acids and ethanol affect the properties of membranes in Drosophila melanogaster larvae.
Long-chain fatty acids and ethanol affect the properties of membranes in Drosophila melanogaster larvae.
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长链脂肪酸和乙醇影响果蝇幼虫的膜特性。
DOI：
10.1007/bf02399919
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发表时间：
1993
影响因子：
2.4
通讯作者：
Geer,BW
中科院分区：
文献类型：
--
作者：
MillerJr,RR;Dare,AO;Moore,ML;Kooser,RG;Geer,BW
本文测定了三个耐乙醇果蝇品系幼虫中性脂和膜脂的脂肪酸组成。膳食乙醇促进中性脂质中长链脂肪酸的减少，沿着所有菌株中乙醇脱氢酶（EC 1.1.1.1）活性的增强。膳食乙醇也增加了14 C-乙醇纳入脂肪酸乙酯（FAEE）的2 - 3倍，并减少了14 C-乙醇纳入游离脂肪酸（FFA）。当培养在无菌的，确定的培养基与硬脂酸在0至5 mM，硬脂酸降低ADH活性高达33%。在菌株没有选择上级耐受乙醇，饮食乙醇促进损失的长链脂肪酸膜脂。在膜中的长链脂肪酸的损失与线粒体膜的疏水结构域中的流动性增加密切相关，通过电子自旋共振测定，并与乙醇耐受性的损失相关。在耐乙醇E2菌株，这已经暴露于乙醇的许多代，饮食乙醇未能促进损失的长链脂肪酸膜脂。
The larval fatty acid composition of neutral lipids and membrane lipids was determined in three ethanol-tolerant strains ofDrosophila melanogaster. Dietary ethanol promoted a decrease in long-chain fatty acids in neutral lipids along with enhanced alcohol dehydrogenase (EC 1.1.1.1) activity in all of the strains. Dietary ethanol also increased the incorporation of14C-ethanol into fatty acid ethyl esters (FAEE) by two- to threefold and decreased the incorporation of14C-ethanol into free fatty acids (FFA). When cultured on sterile, defined media with stearic acid at 0 to 5 mM, stearic acid decreased ADH activity up to 33%. In strains not selected for superior tolerance to ethanol, dietary ethanol promoted a loss of long-chain fatty acids in membrane lipids. The loss of long-chain fatty acids in membranes was strongly correlated with increased fluidity in hydrophobic domains of mitochondrial membranes as determined by electron spin resonance and correlated with a loss of ethanol tolerance. In the ethanol-tolerant E2 strain, which had been exposed to ethanol for many generations, dietary ethanol failed to promote a loss of long-chain fatty acids in membrane lipids.