Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars
Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars
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DOI：
10.1007/s00122-008-0833-0
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发表时间：
2008-10-01
影响因子：
5.4
通讯作者：
Yano, M.
中科院分区：
文献类型：
--
作者：
Matsubara, K.;Kono, I.;Yano, M.
粳稻品种“日本晴”和“越光”在抽穗期和抽穗对光周期的响应（光周期敏感性）方面存在差异。使用简单序列重复 (SSR) 和单核苷酸多态性 (SNP) 标记，我们对日本晴和越光杂交的一组相互回交自交系 (BIL) 中的抽穗日期进行了数量性状位点 (QTL) 分析。在自然日条件下，在两个 BIL 种群中都观察到抽穗期早和晚抽穗期的越界分离。 QTL 分析显示，3 号和 6 号染色体上的两个 QTL 与亲本品种之间抽穗日期的差异有关。 3 号和 6 号染色体 QTL 上的日本晴等位基因分别显示出对两个 BIL 群体中 DTH 的增加和减少作用。 BIL 中观察到的越界分离主要是由一组具有相反作用的等位基因的互补作用造成的。两个 QTL 均被精细定位为二次定位群体（BC2F2 植物/BC2F3 品系）中的单个孟德尔因子。 3 号染色体上的 QTL 定位在 94O03-4 (SSR) 和 OJ21G19-4 (SNP) 之间的 1,140 kb 间隔内，并命名为 Hd16。 6 号染色体上的 QTL 定位在 P548D347 (SSR) 和 0007O20 (SSR) 之间的 328 kb 间隔内，并命名为 Hd17。通过对日本晴近等基因系在短日照和长日照条件下的 DTH 观察发现，Hd16 和 Hd17 都与光周期敏感性有关，这表明 Hd16 和 Hd17 的等位基因差异是亲本品种之间光周期敏感性差异的主要原因。
The rice japonica cultivars Nipponbare and Koshihikari differ in heading date and response of heading to photoperiod (photoperiod sensitivity). Using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers, we conducted quantitative trait locus (QTL) analyses for heading date in a set of reciprocal backcross inbred lines (BILs) from crosses between Nipponbare and Koshihikari. Under natural-day conditions, transgressive segregation in days to heading (DTH) toward both early and late heading was observed in both BIL populations. QTL analyses revealed that two QTLs-on chromosomes 3 and 6-were involved in the difference in heading date between the parental cultivars. The Nipponbare allele at the QTLs on chromosomes 3 and 6 showed, respectively, increasing and decreasing effects on DTH in both BIL populations. The transgressive segregation observed in the BILs could be accounted for mainly by the complementary action of a set of alleles with opposing effects. Both QTLs were finely mapped as single Mendelian factors in secondary mapping populations (BC2F2 plants/BC2F3 lines). The QTL on chromosome 3 was mapped in the 1,140-kb interval between 94O03-4 (SSR) and OJ21G19-4 (SNP) and was designated Hd16. The QTL on chromosome 6 was mapped in the 328-kb interval between P548D347 (SSR) and 0007O20 (SSR) and was designated Hd17. Both Hd16 and Hd17 were involved in photoperiod sensitivity, as revealed by observation of the DTH of nearly isogenic lines of Nipponbare under short- and long-day conditions, suggesting that allelic differences in both Hd16 and Hd17 account for most of the difference in photoperiod sensitivity between the parental cultivars.