WHOLE CHOLERA-TOXIN AND B-SUBUNIT ACT SYNERGISTICALLY AS AN ADJUVANT FOR THE MUCOSAL IMMUNE-RESPONSE OF MICE TO KEYHOLE LIMPET HEMOCYANIN
WHOLE CHOLERA-TOXIN AND B-SUBUNIT ACT SYNERGISTICALLY AS AN ADJUVANT FOR THE MUCOSAL IMMUNE-RESPONSE OF MICE TO KEYHOLE LIMPET HEMOCYANIN
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DOI：
10.1111/j.1365-3083.1990.tb02791.x
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发表时间：
1990-04-01
影响因子：
3.7
通讯作者：
STOKES, CR
中科院分区：
文献类型：
--
作者：
WILSON, AD;CLARKE, CJ;STOKES, CR
霍乱毒素（CT）在口服时是一种有效的IgA反应刺激物，并且已被证明，如果同时给药，它可以促进IgA对第二种蛋白质的反应，如锁孔帽贝血青素（KLH）。在本文中，我们表明，当饲喂5毫克KLH与任何0.5 .mu。g CT或10亩。g B亚基不能刺激粘膜IgA对KLH的反应，喂养0.5 .mu。g CT和10 .mu。g B亚基与5 mg KLH一起产生的局部IgA抗KLH反应与10 .mu产生的反应一样大。整个CT的g。除了刺激固有层的IgA反应外，初步结果表明细胞反应也受到刺激，因为我们已经证明了从喂食10 .mu的小鼠组中分离出的KHL抗原驱动的细胞增殖。g CT + 5mg KLH或0.5 μ。g CT + 10 .mu。g CTB + 5 mg KLH，但单独喂食KLH或10 .mu。g CTB或0.5亩。g CT。这些结果表明，CT的粘膜佐剂作用是由于GM1结合B亚基和a亚基激活腺苷酸环化酶的协同作用。
Cholera toxin (CT) is a potent stimulator of IgA responses when administered orally and has been shown to promote IgA responses to a second protein such as keyhole limpet haemocyanin (KLH) if this is fed simultaneously. In this paper we show that whilst feeding 5 mg KLH with either 0.5 .mu.g CT or 10 .mu.g B subunit fails to stimulate a mucosal IgA response to KLH, feeding 0.5 .mu.g CT and 10 .mu.g B subunit together with 5 mg KLH produces a local IgA anti-KLH response as great as that produced by 10 .mu.g of whole CT. In addition to stimulating IgA responses in the lamina propria, preliminary results indicate that cellular responses are also stimulated, as we have demonstrated KHL antigen-driven proliferation of cells isolated from groups of mice fed either 10 .mu.g CT + 5 mg KLH or 0.5 .mu.g CT + 10 .mu.g CTB + 5 mg KLH but not mice fed KLH alone or with either 10 .mu.g CTB or 0.5 .mu.g CT. These results indicate that the mucosal adjuvant action of CT is due to a synergistic effect involving both the GM1 binding of the B subunit and adenylate cyclase activation by the A subunit.