Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond
Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond
复制标题
用诱导多能干细胞模拟人类疾病：从2D到3D及更远
DOI：
10.1242/dev.156166
复制
发表时间：
2018-03-01
期刊：
影响因子：
4.6
通讯作者：
Wu, Joseph C.
中科院分区：
文献类型：
--
作者：
Liu, Chun;Oikonomopoulos, Angelos;Wu, Joseph C.
人类诱导多能干细胞（iPSC）的出现为人类疾病建模提供了前所未有的机会。在二维（2D）单层中源自iPSC的分化细胞已被证明是探索疾病发病机制和潜在机制的相对简单的工具。在这篇聚焦文章中，我们讨论了当前2D iPSC疾病建模平台的进展和局限性，以及在三维（3D）水平上模拟体内组织和器官的人类iPSC模型开发的最新进展。最近的生物工程方法已开始将不同的3D类器官类型联合收割机组合成单个“4D多器官系统”。我们总结了这种方法的优点，并推测4D多器官系统在人类疾病建模中的未来作用。
The advent of human induced pluripotent stem cells (iPSCs) presents unprecedented opportunities to model human diseases. Differentiated cells derived from iPSCs in two-dimensional (2D) monolayers have proven to be a relatively simple tool for exploring disease pathogenesis and underlying mechanisms. In this Spotlight article, we discuss the progress and limitations of the current 2D iPSC disease-modeling platform, as well as recent advancements in the development of human iPSC models that mimic in vivo tissues and organs at the three-dimensional (3D) level. Recent bioengineering approaches have begun to combine different 3D organoid types into a single '4D multi-organ system'. We summarize the advantages of this approach and speculate on the future role of 4D multi-organ systems in human disease modeling.