DETECTION OF EBER-1 RNA IN PRIMARY BRAIN LYMPHOMAS IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS
DETECTION OF EBER-1 RNA IN PRIMARY BRAIN LYMPHOMAS IN IMMUNOCOMPETENT AND IMMUNOCOMPROMISED PATIENTS
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DOI：
10.1007/bf01057960
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发表时间：
1994-01-01
影响因子：
3.9
通讯作者：
HOCHBERG, F
中科院分区：
文献类型：
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作者：
BASHIR, R;MCMANUS, B;HOCHBERG, F
研究了5例获得性免疫缺陷综合征（AIDS）患者、9例无免疫缺陷患者和2个eb病毒（EBV）阳性对照细胞株（B95-8和Raji）发生的原发性中枢神经系统淋巴瘤（PCNSL）组织中EBER-1 RNA的存在。用地高辛标记的正义或反义EBER-1核探针对组织进行杂交。在所有5个aids相关的pcnsl中，均发现与EBER-1反义探针有强烈的杂交信号。用rna - a对组织进行预孵育可以消除信号。与EBER-1传感探针杂交未见信号。所有免疫正常患者的PCNSLs（5例石蜡包埋，4例冷冻）与EBER-1义或反义探针均无杂交信号，但与免疫球蛋白kappa或lambda轻链探针的杂交信号良好，表明RNA保存。石蜡包埋的b95 -8阳性对照细胞系与反义EBER-1探针的杂交大部分细胞呈阳性，高达1%的细胞与反义EBER-1探针有弱信号。大多数Raji细胞仅用反义EBER-1探针显示出均匀的信号。我们的结论是，艾滋病患者中出现的PCNSLs与潜伏的EBV感染有关，而免疫功能正常的患者的PCNSLs并不表明EBV在这些肿瘤的发病机制中可能起作用。
Tissue from primary central nervous system lymphoma (PCNSL) which developed in five patients with acquired immune deficiency syndrome (AIDS), nine patients without immunodeficiency, and two Epstein-Barr virus (EBV)-positive control cell lines (B95-8 and Raji) were examined for the presence of EBER-1 RNA. The tissues were hybridized with digoxigenin-labeled sense or anti-sense EBER-1 riboprobes. In all five AIDS-related PCNSLs, strong hybridization signals were found with the EBER-1 anti-sense probe. Signals could be eliminated by preincubation of the tissues with RNase-A. Hybridization with the EBER-1 sense probe showed no signal. All PCNSLs from immunocompetent patients (five paraffin-embedded, four frozen) showed no hybridization signals with EBER-1 sense or antisense probe but good hybridization signals with probes to immunoglobulin kappa or lambda light chain indicating RNA preservation. The paraffin-embedded B95-8-positive control cell-line showed positive hybridization in most cells with the anti-sense EBER-1 probe, and up to one percent of the cells had a weak signal with the sense probe. Most Raji cells showed a uniform signal with the anti-sense EBER-1 probe only. We conclude that, PCNSLs that arise in AIDS patients are associated with latent EBV infections, whereas PCNSLs from immunocompetent patients are not indicating a probable role for EBV in pathogenesis of these tumors.