REPLICATION OF ORYCTES BACULOVIRUS IN CELL-CULTURE - VIRAL MORPHOGENESIS, INFECTIVITY AND PROTEIN-SYNTHESIS
REPLICATION OF ORYCTES BACULOVIRUS IN CELL-CULTURE - VIRAL MORPHOGENESIS, INFECTIVITY AND PROTEIN-SYNTHESIS
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DOI：
10.1099/0022-1317-66-3-529
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
SHEEHAN, C
中科院分区：
文献类型：
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作者：
CRAWFORD, AM;SHEEHAN, C
大麦杆状病毒在DSIR-HA-1179细胞中复制的一系列事件始于质膜吞噬病毒颗粒。感染后7h，在细胞核内可见被包裹的病毒颗粒。病毒通过质膜萌发从细胞中释放出来，在质膜上获得第二单位膜。从细胞内纯化的单个包膜的病毒颗粒的感染力与通过发芽获得第二个包膜的病毒颗粒的感染性没有显著差异。用梯度聚丙烯酰胺凝胶电泳法鉴定了27种病毒结构蛋白。用L-[35S]蛋氨酸脉冲标记感染细胞，检测8种主要结构蛋白的合成。在C亚群杆状病毒中没有观察到A亚群杆状病毒的晚期蛋白质合成。
The sequence of events in the replication of Oryctes baculovirus in DSIR-HA-1179 cells began with the uptake of virus particles by pinocytosis at the plasma membrane. Seven h after infection, enveloped virus particles were observed inside a cleared area in the nucleus. Virus was released from cells by budding through the plasma membrane where a second unit membrane was acquired. The infectivity of virus particles with a single envelope purified from within cells was not significantly different from that of virus which had acquired the second envelope by budding. Twenty-seven structural virus proteins were identified by gradient polyacrylamide gel electrophoresis. The synthesis of 8 of the major structural proteins was detected by pulse labeling infected cells with L-[35S]methionine. No late protein synthesis, as observed in subgroup A baculoviruses, was detected in this subgroup C baculovirus.