Melatonin protects against ischemia/reperfusion‐induced oxidative damage to mitochondria in fetal rat brain
Melatonin protects against ischemia/reperfusion‐induced oxidative damage to mitochondria in fetal rat brain
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褪黑素可防止胎鼠脑中缺血/再灌注引起的线粒体氧化损伤
DOI：
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发表时间：
2001
影响因子：
10.3
通讯作者：
Takao Fukaya
中科院分区：
文献类型：
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作者：
A. Wakatsuki;Y. Okatani;K. Shinohara;N. Ikenoue;Takao Fukaya
我们研究了褪黑素对缺血/再灌注诱导的胎鼠脑线粒体氧化损伤的影响。在妊娠第19天，将雌性Wistar大鼠的子宫卵巢动脉双侧闭塞20分钟，以诱导胎仔缺血。再灌注通过释放闭塞和恢复循环30 min来实现。对照组大鼠进行假手术。在闭塞前60分钟腹膜内注射褪黑激素（10 mg/kg）或载体。我们测量了呼吸控制指数（RCI）和5-二磷酸腺苷（ADP）/氧比作为线粒体呼吸活性的指标，以及胎脑线粒体中硫代巴比妥酸反应物质（TBARS）的浓度。缺血/再灌注显著升高TBARS浓度，显著降低RCI以及ADP/氧比。褪黑激素治疗逆转了缺血/再灌注诱导的RCI降低（2.29±0.06-2.64±0.09，P<0.05），ADP/O2比值（1.48±0.03-1.57±0.02，P<0.05），并能降低TBARS浓度的升高（11.00±0.34-7.57±0.74 nM/mg蛋白，P<0.01），导致与未治疗的假缺血动物相似的值。结果表明，妊娠大鼠给予褪黑激素可预防缺血/再灌注诱导的胎鼠脑中的氧化线粒体损伤。
We investigated the effects of melatonin on ischemia/reperfusion‐induced oxidative damage to mitochondria in fetal rat brain. The utero‐ovarian arteries were occluded bilaterally for 20 min in female Wistar rats on day 19 of pregnancy to induce fetal ischemia. Reperfusion was achieved by releasing the occlusion and restoring circulation for 30 min. A sham operation was performed in control rats. Melatonin (10 mg/kg) or vehicle was injected intraperitoneally 60 min prior to occlusion. We measured the respiratory control index (RCI) and the adenosine 5‐diphosphate (ADP)/oxygen ratio as indicators of mitochondrial respiratory activity, as well as the concentration of thiobarbituric acid‐reactive substances (TBARS) in the mitochondria of fetal brain. Ischemia/reperfusion significantly elevated the concentration of TBARS and significantly reduced the RCI as well as the ADP/oxygen ratio. Melatonin treatment reversed the ischemia/reperfusion‐induced reductions in the RCI (2.29±0.06–2.64±0.09, P<0.05) and in the ADP/oxygen ratio (1.48±0.03–1.57±0.02, P<0.05), and also reduced the elevation in concentration of TBARS (11.00±0.34–7.57±0.74 nM/mg protein, P<0.01), resulting in values similar to those in untreated, sham‐ischemic animals. The results indicate that administration of melatonin to pregnant rats may prevent ischemia/reperfusion‐induced oxidative mitochondrial damage in fetal rat brain.
胎儿豚鼠大脑在发育过程中的抗氧化酶和母体缺氧的影响。
影响因子：
2.9
作者：
Mishra,OP;Delivoria-Papadopoulos,M
通讯作者：
Delivoria-Papadopoulos,M