Soybean late embryogenesis abundant protein GmLEA4 interacts with GmCaM1, enhancing seed vigor in transgenic Arabidopsis under high temperature and humidity stress
Soybean late embryogenesis abundant protein GmLEA4 interacts with GmCaM1, enhancing seed vigor in transgenic Arabidopsis under high temperature and humidity stress
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DOI：
10.1007/s10725-022-00930-w
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发表时间：
2022-11
影响因子：
4.2
通讯作者：
Yingzi Shen;Jiaping Wei;Yali Zhou;Yajing Zhu;Sushuang Liu;Yilong Wang;Hao Ma
中科院分区：
文献类型：
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作者：
Yingzi Shen;Jiaping Wei;Yali Zhou;Yajing Zhu;Sushuang Liu;Yilong Wang;Hao Ma
钙调素（CaM）是一种普遍存在的钙离子结合蛋白，参与多种信号转导过程，在所有真核细胞中都有表达，而胚胎发育后期丰富蛋白（莱亚）是一个大家族的亲水性蛋白，在发育过程中积累，以保护种子免受水分胁迫。在我们前期的研究中，GmLEA4是通过酵母双杂交技术筛选到的一个与GmCaM1相互作用的候选蛋白，而GmCaM1与高温高湿胁迫下大豆种子活力的形成密切相关。在本研究中，GmLEA4定位于细胞膜和细胞核。酵母双杂交、双分子荧光互补（BiFC）和GST pull down进一步证实了GmCaM1和GmLEA4之间的相互作用，GmLEA4和GmCaM1在种子中的表达量高于其他组织，并参与了对HTH胁迫的响应。GmCaM1和GmLEA4在拟南芥中的过表达提高了种子清除ROS的能力，提高了HTH胁迫下种子的活力。结果表明，GmLEA4与GmCaM1相互作用，可能参与了HTH胁迫下大豆种子活力的形成。
Calmodulin (CaM) as a ubiquitous Ca2+-binding protein is involved in multiple signaling processes expressed in all the eukaryotic cells, while the late embryogenesis abundant (LEA) proteins are a large family of hydrophilic proteins that accumulate in developing seeds to ensure protection against water deficit stress. In our previous study, GmLEA4 was screened as a candidate GmCaM1-interacting protein by yeast two-hybrid assay while GmCaM1 was found to be highly related to the formation of seed vigor under high temperature and humidity (HTH) stress in soybean. In the present study, GmLEA4 was localized on the cell membrane and nucleus. The interaction between GmCaM1 and GmLEA4 was further confirmed by yeast two-hybrid assay, bimolecular fluorescence complementation (BiFC) and GST pull down.GmLEA4andGmCaM1showed higher expression levels in seeds than in other tissues and were involved in response to HTH stress. The overexpression ofGmCaM1andGmLEA4inArabidopsisraised the ROS scavenging ability in seeds and improved seed vitality under HTH stress. Our results indicated that GmLEA4 interacts with GmCaM1, maybe participating in seed vigor formation under HTH stress in soybean.